Product Name
PNKP/PNK, Polyclonal Antibody
Popular Item
Full Product Name
Goat anti-PNKP/PNK Antibody
Product Synonym Names
PNKP; polynucleotide kinase 3'-phosphatase; PNK; polynucleotide kinase 3-prime-phosphatase; Homo sapiens polynucleotide kinase 3'-phosphatase (PNKP); PNKP antibody; polynucleotide kinase 3\1-phosphatase antibody; PNK antibody; polynucleotide kinase 3-prime-phosphatase antibody; Homo sapiens polynucleotide kinase 3\1-phosphatase (PNKP) antibody; PNKP / PNK
Product Gene Name
anti-PNKP antibody
[Similar Products]
Product Synonym Gene Name
PNK[Similar Products]
Antibody/Peptide Pairs
PNKP / PNK peptide (MBS425278) is used for blocking the activity of PNKP/PNK antibody (MBS420796)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
EPRLGRLYCQ FSEG
Species Reactivity
Tested: Human; Expected from sequence similarity: Human
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence EPRLGRLYCQFSEG, from the C Terminus of the protein sequence according to NP_009185.2.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-PNKP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-PNKP antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-PNKP antibody
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Sucessfully used to detect recombinant human PNKP (1/1000 dilution).
NCBI/Uniprot data below describe general gene information for PNKP. It may not necessarily be applicable to this product.
NCBI Accession #
NP_009185.2
[Other Products]
NCBI GenBank Nucleotide #
NM_007254.3
[Other Products]
UniProt Secondary Accession #
Q9BUL2; Q9P1V2; Q9UKU8; Q9UNF8; Q9UNI0[Other Products]
UniProt Related Accession #
Q96T60[Other Products]
Molecular Weight
53,004 Da
NCBI Official Full Name
bifunctional polynucleotide phosphatase/kinase
NCBI Official Synonym Full Names
polynucleotide kinase 3'-phosphatase
NCBI Official Symbol
PNKP [Similar Products]
NCBI Official Synonym Symbols
PNK; AOA4; MCSZ; EIEE10
[Similar Products]
NCBI Protein Information
bifunctional polynucleotide phosphatase/kinase
UniProt Protein Name
Bifunctional polynucleotide phosphatase/kinase
UniProt Synonym Protein Names
DNA 5'-kinase/3'-phosphatase; Polynucleotide kinase-3'-phosphataseIncluding the following 2 domains:Polynucleotide 3'-phosphatase (EC:3.1.3.32)Alternative name(s):2'(3')-polynucleotidase
UniProt Gene Name
PNKP [Similar Products]
UniProt Entry Name
PNKP_HUMAN
NCBI Summary for PNKP
This locus represents a gene involved in DNA repair. In response to ionizing radiation or oxidative damage, the protein encoded by this locus catalyzes 5' phosphorylation and 3' dephosphorylation of nucleic acids. Mutations at this locus have been associated with microcephaly, seizures, and developmental delay.[provided by RefSeq, Sep 2010]
UniProt Comments for PNKP
Pnk1: polynucleotide kinase 3'-phosphatase. May be involved in double-strand break repair by non-homologous end joining. Required for normal response to DNA damage by gamma-radiation or camptothecin in S. pombe.
Protein type: Phosphatase (non-protein); EC 2.7.1.78; DNA-binding; EC 3.1.3.32; Kinase, other
Chromosomal Location of Human Ortholog: 19q13.3-q13.4
Cellular Component: membrane; nucleolus; nucleoplasm; nucleus
Molecular Function: ATP binding; ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity; damaged DNA binding; double-stranded DNA binding; endonuclease activity; nucleotide kinase activity; polynucleotide 3'-phosphatase activity; protein binding; purine nucleotide binding
Biological Process: dephosphorylation; DNA damage response, detection of DNA damage; DNA repair; DNA-dependent DNA replication; nucleotide phosphorylation; nucleotide-excision repair, DNA damage removal; positive regulation of telomerase activity; positive regulation of telomere maintenance via telomerase; response to oxidative stress; response to radiation
Disease: Ataxia-oculomotor Apraxia 4; Microcephaly, Seizures, And Developmental Delay
Research Articles on PNKP
1. Here we report that purified wild-type (WT) ATXN3 stimulates, and by contrast the mutant form specifically inhibits, PNKP's 3' phosphatase activity in vitro. ATXN3-deficient cells also show decreased PNKP activity
Precautions
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Disclaimer
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