MGAT1, Polyclonal Antibody

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Small volumes of anti-MGAT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Polyclonal

Western Blot (WB), Immunohistochemistry (IHC)

Western blot analysis of extracts of various cell lines, using MGAT1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human liver injury using MGAT1 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using MGAT1 antibody at dilution of 1:100 (40x lens).

445

mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase

alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase; glcNAc-T I; N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I

Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase

GGNT1; GLCT1; GLYT1; MGAT; GNT-I; GlcNAc-T I  [Similar Products]

MGAT1_HUMAN

There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I is a medial-Golgi enzyme essential for the synthesis of hybrid and complex N-glycans. The protein, encoded by a single exon, shows typical features of a type II transmembrane protein. The protein is believed to be essential for normal embryogenesis. Several variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

MGAT1: Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans. Belongs to the glycosyltransferase 13 family.

Protein type: Glycan Metabolism - N-glycan biosynthesis; EC 2.4.1.101; Membrane protein, integral; Transferase

Chromosomal Location of Human Ortholog: 5q35

Cellular Component: Golgi membrane; membrane; integral to membrane; vesicle

Molecular Function: acetylglucosaminyltransferase activity; metal ion binding; alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity

Biological Process: cellular protein metabolic process; in utero embryonic development; carbohydrate metabolic process; protein amino acid glycosylation; protein amino acid N-linked glycosylation via asparagine; UDP-N-acetylglucosamine catabolic process; post-translational protein modification

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