Activin Receptor Type IA (ACVR1), Polyclonal Antibody

Activin receptor type-1; Activin receptor type I; ACTR-I; Activin receptor-like kinase 2; ALK-2; Serine/threonine-protein kinase receptor R1; SKR1; TGF-B superfamily receptor type I; TSR-I; ACVR1; ACVRLK2

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This Activin Receptor Type IA (ACVR1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 132-162 amino acids from the Central region of human Activin Receptor Type IA (ACVR1).

Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Vial Concentration: 2 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-ACVR1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I (I and IB) and two type II (II and IIB) receptors. These receptors are all transmembrane proteins, composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic domain with predicted serine/threonine specificity. Type I receptors are essential for signaling; and type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. ACVR1 (activin A type I receptor) signals a particular transcriptional response in concert with activin type II receptors.

Crown Antibodies; Signal Transduction

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)

WB~~1:1000

Western blot analysis of lysate from HepG2 cell line, using ACVR1 Antibody (R147). MBS9208699 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

Western blot analysis of ACVR1 (arrow) using rabbit polyclonal ACVR1 Antibody (R147). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ACVR1 gene.

ACVR1 Antibody (R147) western blot analysis in U937 cell line lysates (35ug/lane).This demonstrates the ACVR1 antibody detected the ACVR1 protein (arrow).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.

57153

activin A receptor, type I

activin receptor type-1

Activin receptor type-1

ACVRLK2; ACTR-I; ALK-2; SKR1; TSR-I  [Similar Products]

ACVR1_HUMAN

Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I ( I and IB) and two type II (II and IIB) receptors. These receptors are all transmembrane proteins, composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic domain with predicted serine/threonine specificity. Type I receptors are essential for signaling; and type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. This gene encodes activin A type I receptor which signals a particular transcriptional response in concert with activin type II receptors. Mutations in this gene are associated with fibrodysplasia ossificans progressive. [provided by RefSeq, Jul 2008]

ALK2: On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for activin. May be involved for left-right pattern formation during embryogenesis. Interacts with FKBP1A. Interacts with FCHO1. Expressed in normal parenchymal cells, endothelial cells, fibroblasts and tumor-derived epithelial cells. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.

Protein type: Kinase, protein; EC 2.7.11.30; Protein kinase, Ser/Thr (receptor); Membrane protein, integral; Protein kinase, TKL; TKL group; STKR family; Type1 subfamily

Chromosomal Location of Human Ortholog: 2q23-q24

Cellular Component: integral to plasma membrane; apical part of cell; activin receptor complex

Molecular Function: protein homodimerization activity; metal ion binding; peptide hormone binding; transmembrane receptor protein serine/threonine kinase activity; protein kinase activity; protein serine/threonine kinase activity; protein binding; activin receptor activity, type I; transforming growth factor beta binding; transforming growth factor beta receptor activity, type I; activin binding; SMAD binding; ATP binding; receptor signaling protein serine/threonine kinase activity

Biological Process: pharyngeal system development; in utero embryonic development; positive regulation of transcription, DNA-dependent; peptidyl-threonine phosphorylation; activin receptor signaling pathway; gastrulation with mouth forming second; positive regulation of bone mineralization; protein amino acid phosphorylation; negative regulation of signal transduction; germ cell development; patterning of blood vessels; BMP signaling pathway; positive regulation of osteoblast differentiation; negative regulation of activin receptor signaling pathway; mesoderm formation; transforming growth factor beta receptor signaling pathway; smooth muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; regulation of skeletal muscle development; regulation of ossification; determination of left/right symmetry; neural crest cell migration; acute inflammatory response; urogenital system development; G1/S transition of mitotic cell cycle

Disease: Fibrodysplasia Ossificans Progressiva

Schneider-Kolsky, M.E., et al., Placenta 23(4):294-302 (2002).
Roijer, E., et al., Mamm. Genome 9(3):266-268 (1998).
Attisano, L., et al., Cell 75(4):671-680 (1993).
ten Dijke, P., et al., Oncogene 8(10):2879-2887 (1993).
Matsuzaki, K., et al., J. Biol. Chem. 268(17):12719-12723 (1993).

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