ABCB1 / MDR1 / P Glycoprotein, Polyclonal Antibody

Anti-ABCB1 / MDR1 / P Glycoprotein Antibody (aa262-277) IHC-plus; ABCB1; ABC20; Abcb1b; CLCS; Colchicin sensitivity; CD243; Doxorubicin resistance; IBD13; gp170; MDR1; Multidrug resistance protein 1; P glycoprotein; P-glycoprotein 1; P-GP; CD243 antigen; PGY1; Human ABCB1; MDR1; P Glycoprotein

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Amino acids 262-277 of human ABCB1 protein.

Immunoaffinity Purified

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.

1 mg/ml (lot specific)

Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.

Small volumes of anti-ABCB1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

ABCB1/MDR1/P Glycoprotein is an ATP dependent transporter that mediates efflux of chemotherapeutic agents from the intracellular milieu. P Glycoprotein has also been associated with volume-regulated chloride channel activity and basal to apical transendothelial migration of mononuclear phagocytes.

Subfamily:-ATP-binding-cassette---ABCB/MDR">Family: Transporter
Subfamily: ATP-binding cassette - ABCB/MDR

Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)

ELISA (1:10000 - 1:50000), IHC-P (5 ug/ml), WB (1:200 - 1:2000)
Usage: Tested in Western blot with recombinant proteins only. Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6....

Anti-MDR1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Anti-ABCB1 Antibody - Western Blot. Western blot of affinity purified anti-ABCB1 antibody shows detection of ABCB1 in crude membrane extracts from HF insect cells over-expressing human ABCB1. The extract was loaded onto a gel in the amounts indicated followed by electrophoresis and transfer to nitrocellulose. The membrane was probed with the primary antibody diluted to 1:600, followed by Peroxidase Conjugated Affinity Purified Anti-RABBIT IgG at 1:10000. Personal Communication, Anna Calcagno, CCR-NCI, Bethesda, MD.

134,032 Da

ATP-binding cassette, sub-family B (MDR/TAP), member 1

multidrug resistance protein 1; P-glycoprotein 1; colchicin sensitivity; doxorubicin resistance

Multidrug resistance protein 1

MDR1; PGY1  [Similar Products]

MDR1_HUMAN

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. [provided by RefSeq, Jul 2008]

ABCB1: Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells. Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. Interacts with PSMB8.

Protein type: Cell adhesion; Transporter, ABC family; Hydrolase; Membrane protein, multi-pass; EC 3.6.3.44; Transporter; Membrane protein, integral

Chromosomal Location of Human Ortholog: 7q21.12

Cellular Component: cell surface; membrane; integral to membrane; plasma membrane

Molecular Function: protein binding; transporter activity; ATPase activity, coupled to transmembrane movement of substances; ATP binding; xenobiotic-transporting ATPase activity

Biological Process: response to drug; transport; xenobiotic transport; multidrug transport; G2/M transition of mitotic cell cycle; transmembrane transport

Disease: Colchicine Resistance; Inflammatory Bowel Disease 13

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