Fast skeletal troponin T (fast skTnT), Recombinant Protein

Troponin T skeletal, fast, human, recombinant

For Research Use Only. Not for use in diagnostic procedures.

Expressed in E Coli

> 95 % (SDS-PAGE)
lon-exchange chromatography

Lyophilized from 0.01 M HCl, pH 2.0

Store at -20 degree C in lyophilized form (-15 to -30 degree C allowed)
-70 degree C after reconstitution (-65 to -80 degree C allowed), max. 5 days at -20 degree C (-15 to -30 degree C allowed)
Avoid repeated freezing and thawing.
It is recommended to aliquot the product after reconstitution.

Small volumes of skTnT recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

261 a.a.r.
Theoretical molecular weight (calculated from the sequence) 30 964 Da.
Does not contain tags
Theoretical pI 6.07

Biochemical studies, mass fast skTnT standard, immunoassay standard and calibrator, immunogen for antibody production.

30,000 Da

troponin T3, fast skeletal type

troponin T, fast skeletal muscle

Troponin T, fast skeletal muscle

TnTf; fTnT  [Similar Products]

TNNT3_HUMAN

The binding of Ca(2+) to the trimeric troponin complex initiates the process of muscle contraction. Increased Ca(2+) concentrations produce a conformational change in the troponin complex that is transmitted to tropomyosin dimers situated along actin filaments. The altered conformation permits increased interaction between a myosin head and an actin filament which, ultimately, produces a muscle contraction. The troponin complex has protein subunits C, I, and T. Subunit C binds Ca(2+) and subunit I binds to actin and inhibits actin-myosin interaction. Subunit T binds the troponin complex to the tropomyosin complex and is also required for Ca(2+)-mediated activation of actomyosin ATPase activity. There are 3 different troponin T genes that encode tissue-specific isoforms of subunit T for fast skeletal-, slow skeletal-, and cardiac-muscle. This gene encodes fast skeletal troponin T protein; also known as troponin T type 3. Alternative splicing results in multiple transcript variants encoding additional distinct troponin T type 3 isoforms. A developmentally regulated switch between fetal/neonatal and ***** troponin T type 3 isoforms occurs. Additional splice variants have been described but their biological validity has not been established. Mutations in this gene may cause distal arthrogryposis multiplex congenita type 2B (DA2B). [provided by RefSeq, Oct 2009]

TNNT3: Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Defects in TNNT3 are a cause of distal arthrogryposis type 2B (DA2B); also known as arthrogryposis multiplex congenita, distal, type 2B (AMCD2B). DA2B is a form of inherited multiple congenital contractures. Affected individuals have vertical talus, ulnar deviation in the hands, severe camptodactyly, and a distinctive face characterized by a triangular shape, prominent nasolabial folds, small mouth and a prominent chin. Belongs to the troponin T family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Motor

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: cytosol; troponin complex

Molecular Function: actin binding; calcium-dependent ATPase activity; calcium-dependent protein binding; tropomyosin binding; troponin C binding; troponin I binding

Biological Process: muscle filament sliding; regulation of ATPase activity; regulation of striated muscle contraction; skeletal muscle contraction

Disease: Arthrogryposis, Distal, Type 2b

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