Product Name
FANCD2, Polyclonal Antibody
Full Product Name
FANCD2 (Phospho-Ser222) Antibody
Product Synonym Names
FACD2; FANCD2; Fanconi anemia complementation group D2 protein
Product Gene Name
anti-FANCD2 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9BXW9
Species Reactivity
Human, Mouse, Rat
Specificity
FANCD2 (Phospho-Ser222) Antibody detects endogenous levels of FANCD2 only when phosphorylated at Ser222.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (lot specific)
Target Modification
Phospho
Modification Sites
H:S222; M:S220; R:S222
Immunogen
The antiserum was produced against synthesized peptide derived from human FANCD2 around the phosphorylation site of Ser222.
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Other Notes
Small volumes of anti-FANCD2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-FANCD2 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-FANCD2 antibody
WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:1000
Immunohistochemistry (IHC) of anti-FANCD2 antibody
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using FANCD2 (Phospho-Ser222) Antibody. The picture on the right is blocked with the phospho peptide.
Western Blot (WB) of anti-FANCD2 antibody
Western blot analysis of lysates from HT29 cells treated with Calyculin A 50ng/ml 30', using FANCD2 (Phospho-Ser222) Antibody. The lane on the right is blocked with the phospho peptide.
NCBI/Uniprot data below describe general gene information for FANCD2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001018125.1
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NCBI GenBank Nucleotide #
NM_001018115.2
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UniProt Primary Accession #
Q9BXW9
[Other Products]
UniProt Secondary Accession #
Q2LA86; Q69YP9; Q6PJN7; Q9BQ06; Q9H9T9[Other Products]
UniProt Related Accession #
Q9BXW9[Other Products]
NCBI Official Full Name
Fanconi anemia group D2 protein isoform b
NCBI Official Synonym Full Names
Fanconi anemia complementation group D2
NCBI Official Symbol
FANCD2 [Similar Products]
NCBI Official Synonym Symbols
FA4; FAD; FACD; FAD2; FA-D2; FANCD
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NCBI Protein Information
Fanconi anemia group D2 protein
UniProt Protein Name
Fanconi anemia group D2 protein
Protein Family
FANCD2 opposite strand protein
UniProt Gene Name
FANCD2 [Similar Products]
UniProt Synonym Gene Names
FACD; Protein FACD2 [Similar Products]
NCBI Summary for FANCD2
The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group D2. This protein is monoubiquinated in response to DNA damage, resulting in its localization to nuclear foci with other proteins (BRCA1 AND BRCA2) involved in homology-directed DNA repair. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]
UniProt Comments for FANCD2
Required for maintenance of chromosomal stability. Promotes accurate and efficient pairing of homologs during meiosis. Involved in the repair of DNA double-strand breaks, both by homologous recombination and single-strand annealing. May participate in S phase and G2 phase checkpoint activation upon DNA damage. Plays a role in preventing breakage and loss of missegregating chromatin at the end of cell division, particularly after replication stress. Required for the targeting, or stabilization, of BLM to non-centromeric abnormal structures induced by replicative stress. Promotes BRCA2/FANCD1 loading onto damaged chromatin. May also be involved in B-cell immunoglobulin isotype switching.
Research Articles on FANCD2
1. FANCD2 has a ubiquitination-independent role in countering endogenous levels of replication stress, a function that is critical for the maintenance of genomic stability.
Precautions
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Disclaimer
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