CCNH, Polyclonal Antibody

Cyclin-H; MO15-associated protein; p34; p37; CCNH

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This CCNH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-52 amino acids from the N-terminal region of human CCNH.

Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Vial Concentration: 2 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-CCNH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

The protein encoded by this gene belongs to the highly
conserved cyclin family, whose members are characterized by a
dramatic periodicity in protein abundance through the cell cycle.
Cyclins function as regulators of CDK kinases. Different cyclins
exhibit distinct expression and degradation patterns which
contribute to the temporal coordination of each mitotic event. This
cyclin forms a complex with CDK7 kinase and ring finger protein
MAT1. The kinase complex is able to phosphorylate CDK2 and CDC2
kinases, thus functions as a CDK-activating kinase (CAK). This
cyclin and its kinase partner are components of TFIIH, as well as
RNA polymerase II protein complexes. They participate in two
different transcriptional regulation processes, suggesting an
important link between basal transcription control and the cell
cycle machinery. A pseudogene of this gene is found on chromosome
4. Alternate splicing results in multiple transcript variants.

Cell Biology; Immunology

Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS)

WB~~1:1000

CCNH Antibody (N-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the CCNH antibody detected the CCNH protein (arrow).

CCNH Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

37643

cyclin H

cyclin-H

Cyclin-H

CCNH_HUMAN

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. The kinase complex is able to phosphorylate CDK2 and CDC2 kinases, thus functions as a CDK-activating kinase (CAK). This cyclin and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes. They participate in two different transcriptional regulation processes, suggesting an important link between basal transcription control and the cell cycle machinery. A pseudogene of this gene is found on chromosome 4. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Nov 2010]

CCNH: Regulates CDK7, the catalytic subunit of the CDK- activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle. Associates primarily with CDK7 and MAT1 to form the CAK complex. CAK can further associate with the core-TFIIH to form the TFIIH basal transcription factor. Belongs to the cyclin family. Cyclin C subfamily.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 5q13.3-q14

Cellular Component: nucleoplasm; holo TFIIH complex; nucleus; cyclin-dependent protein kinase activating kinase holoenzyme complex

Molecular Function: RNA polymerase subunit kinase activity; DNA-dependent ATPase activity; protein binding; cyclin-dependent protein kinase regulator activity; protein kinase binding

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; viral reproduction; positive regulation of viral transcription; transcription from RNA polymerase I promoter; RNA elongation from RNA polymerase I promoter; DNA repair; termination of RNA polymerase I transcription; protein amino acid phosphorylation; regulation of gene expression, epigenetic; mRNA capping; negative regulation of gene expression, epigenetic; nucleotide-excision repair; transcription-coupled nucleotide-excision repair; RNA elongation from RNA polymerase II promoter; gene expression; positive regulation of transcription from RNA polymerase II promoter; nucleotide-excision repair, DNA damage removal; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; G2/M transition of mitotic cell cycle; transcription initiation from RNA polymerase I promoter; G1/S transition of mitotic cell cycle

Guey, L.T., et al. Eur. Urol. 57(2):283-292(2010)
Hosgood, H.D. III, et al. Respir Med 103(12):1866-1870(2009)
Young, R.P., et al. Postgrad Med J 85(1008):515-524(2009)
Kweekel, D.M., et al. Br. J. Cancer 101(2):357-362(2009)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)

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