Product Name
FANCD2, siRNA
Full Product Name
FANCD2 siRNA (Mouse)
Product Synonym Names
Fanconi anemia group D2 protein homolog; Protein FACD2
Product Gene Name
FANCD2 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q80V62
Specificity
FANCD2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse FANCD2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of FANCD2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
FANCD2 sirna
siRNA to inhibit FANCD2 expression using RNA interference
Applications Tested/Suitable for FANCD2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for FANCD2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001028416.2
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NCBI GenBank Nucleotide #
NM_001033244.3
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UniProt Primary Accession #
Q80V62
[Other Products]
UniProt Secondary Accession #
Q9CWC7[Other Products]
UniProt Related Accession #
Q80V62[Other Products]
Molecular Weight
14,683 Da
NCBI Official Full Name
Fanconi anemia group D2 protein homolog
NCBI Official Synonym Full Names
Fanconi anemia, complementation group D2
NCBI Official Symbol
Fancd2 [Similar Products]
NCBI Official Synonym Symbols
FA4; FAD; FACD; FA-D2; FANCD; AU015151; BB137857; 2410150O07Rik
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NCBI Protein Information
Fanconi anemia group D2 protein homolog
UniProt Protein Name
Fanconi anemia group D2 protein homolog
Protein Family
FANCD2 opposite strand protein
UniProt Gene Name
Fancd2 [Similar Products]
UniProt Synonym Gene Names
Protein FACD2 [Similar Products]
UniProt Entry Name
FACD2_MOUSE
UniProt Comments for FANCD2
FANCD2: Required for maintenance of chromosomal stability. Promotes accurate and efficient pairing of homologs during meiosis. Involved in the repair of DNA double-strand breaks, both by homologous recombination and single-strand annealing. May participate in S phase and G2 phase checkpoint activation upon DNA damage. Plays a role in preventing breakage and loss of missegregating chromatin at the end of cell division, particularly after replication stress. Required for the targeting, or stabilization, of BLM to non-centromeric abnormal structures induced by replicative stress. Promotes BRCA2/FANCD1 loading onto damaged chromatin. May also be involved in B-cell immunoglobulin isotype switching. Interacts directly with FANCE and FANCI. Interacts with USP1 and MEN1. The ubiquitinated form specifically interacts with BRCA1 and BLM. Both the nonubiquitinated and the monoubiquitinated forms interact with BRCA2; this interaction is mediated by phosphorylated FANCG and the complex also includes XCCR3. The ubiquitinated form specifically interacts with MTMR15/FAN1 (via UBZ-type zinc finger), leading to recruit MTMR15/FAN1 to sites of DNA damage. Interacts with DCLRE1B/Apollo. Highly expressed in germinal center cells of the spleen, tonsil, and reactive lymph nodes, and in the proliferating basal layer of squamous epithelium of tonsil, esophagus, oropharynx, larynx and cervix. Expressed in cytotrophoblastic cells of the placenta and exocrine cells of the pancreas. Highly expressed in testis, where expression is restricted to maturing spermatocytes. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA repair, damage
Cellular Component: nucleoplasm; Golgi apparatus; intracellular membrane-bound organelle; condensed chromosome; nucleus
Molecular Function: protein binding
Biological Process: multicellular organismal development; gamete generation; synapsis; response to gamma radiation; cell cycle; DNA repair; response to DNA damage stimulus
Research Articles on FANCD2
1. Data demonstrated that Fancd2 was required for nuclear retention of CA-FOXO3a and for maintaining hematopoietic repopulation of the HSCs.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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