Product Name
CDCP1, siRNA
Full Product Name
CDCP1 siRNA (Mouse)
Product Synonym Names
CUB domain-containing protein 1; Membrane glycoprotein gp140; Transmembrane and associated with src kinases; CD antigen CD318
Product Gene Name
CDCP1 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q5U462
Specificity
CDCP1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CDCP1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of CDCP1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CDCP1 sirna
siRNA to inhibit CDCP1 expression using RNA interference
Applications Tested/Suitable for CDCP1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for CDCP1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_598735.2
[Other Products]
NCBI GenBank Nucleotide #
NM_133974.3
[Other Products]
UniProt Primary Accession #
Q5U462
[Other Products]
UniProt Secondary Accession #
Q810J0; Q921M9[Other Products]
UniProt Related Accession #
Q5U462[Other Products]
Molecular Weight
92,726 Da
NCBI Official Full Name
CUB domain-containing protein 1
NCBI Official Synonym Full Names
CUB domain containing protein 1
NCBI Official Symbol
Cdcp1 [Similar Products]
NCBI Official Synonym Symbols
AA409659; 9030022E12Rik; E030027H19Rik
[Similar Products]
NCBI Protein Information
CUB domain-containing protein 1
UniProt Protein Name
CUB domain-containing protein 1
UniProt Synonym Protein Names
Membrane glycoprotein gp140; Transmembrane and associated with src kinases; CD_antigen: CD318
Protein Family
CUB domain-containing protein
UniProt Gene Name
Cdcp1 [Similar Products]
UniProt Entry Name
CDCP1_MOUSE
UniProt Comments for CDCP1
CDCP1: a transmembrane protein containing three extracellular CUB domains involved in cell adhesion and cell matrix association. May play a role in the regulation of anchorage versus migration or proliferation versus differentiation depending upon its phosphorylation status. Interacts with N- and P-cadherin, syndecans 1 and 4, the serine protease ST14, SRC and PKCG. Highly expressed in mitotic cells with low expression during interphase. Detected at highest levels in skeletal muscle and colon with lower levels in kidney, small intestine, placenta and lung. Up-regulated in a number of human tumor cell lines, as well as in colorectal cancer, breast carcinoma and lung cancer. Its expression level is correlated with the metastatic ability of carcinoma cells. May be a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets. Belongs to the tetraspanin web involved in tumor progression and metastasis. Also expressed in cells with phenotypes reminiscent of mesenchymal stem cells and neural stem cells. Three alternatively spliced human isoforms have been reported. Isoform 1 is a single-pass membrane protein that may also be shed, giving rise to a soluble peptide. Isoform 3 is secreted.
Protein type: Membrane protein, integral; Cell surface
Cellular Component: membrane; plasma membrane; integral to membrane
Research Articles on CDCP1
1. Trask loss enhances tumorigenic growth by liberating integrin signaling and growth factor receptor cross-talk in unanchored cells.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
