Product Name
TCAP, Polyclonal Antibody
Popular Item
Full Product Name
TCAP Polyclonal Antibody
Product Synonym Names
CMD1N; CMH25; LGMD2G; T-cap; TELE; telethonin
Product Gene Name
anti-TCAP antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
MATSELSCEV SEENCERREA FWAEWKDLTL STRPEEGCSL HEEDTQRHET YHQQGQCQVL VQRSPWLMMR MGILGRGLQE YQLPYQRVLP LPIFTPAKMG ATKEEREDTP IQLQELLALE TALGGQCVDR QEVAEITKQL PPVVPVSKPG ALRRSLSRSM SQEAQRG
3D Structure
ModBase 3D Structure for O15273
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Affinity Purification
Immunogen
Recombinant protein of human TCAP
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Cellular Location
Cytoplasm, myofibril, sarcomere
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Other Notes
Small volumes of anti-TCAP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-TCAP antibody
Sarcomere assembly is regulated by the muscle protein titin. Titin is a giant elastic protein with kinase activity that extends half the length of a sarcomere. It serves as a scaffold to which myofibrils and other muscle related proteins are attached. This gene encodes a protein found in striated and cardiac muscle that binds to the titin Z1-Z2 domains and is a substrate of titin kinase, interactions thought to be critical to sarcomere assembly. Mutations in this gene are associated with limb-girdle muscular dystrophy type 2G.
Product Categories/Family for anti-TCAP antibody
Polyclonal Antibodies
Applications Tested/Suitable for anti-TCAP antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes for anti-TCAP antibody
WB: 1:500 - 1:2000
IF: 1:50 - 1:200
Western Blot (WB) of anti-TCAP antibody
Western blot analysis of extracts of various cell lines, using TCAP antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Immunofluorescence (IF) of anti-TCAP antibody
Immunofluorescence analysis of HeLa cells using TCAP antibody. Blue: DAPI for nuclear staining.
NCBI/Uniprot data below describe general gene information for TCAP. It may not necessarily be applicable to this product.
NCBI Accession #
NP_003664.1
[Other Products]
NCBI GenBank Nucleotide #
NM_003673.3
[Other Products]
UniProt Primary Accession #
O15273
[Other Products]
UniProt Secondary Accession #
Q96L27[Other Products]
UniProt Related Accession #
O15273[Other Products]
Molecular Weight
Calculated: 19kDa
Observed: 22kDa
NCBI Official Full Name
telethonin
NCBI Official Synonym Full Names
titin-cap
NCBI Official Symbol
TCAP [Similar Products]
NCBI Official Synonym Symbols
TELE; CMD1N; CMH25; T-cap; LGMD2G; telethonin
[Similar Products]
NCBI Protein Information
telethonin
UniProt Protein Name
Telethonin
UniProt Synonym Protein Names
Titin cap protein
Protein Family
Telethonin
UniProt Gene Name
TCAP [Similar Products]
NCBI Summary for TCAP
Sarcomere assembly is regulated by the muscle protein titin. Titin is a giant elastic protein with kinase activity that extends half the length of a sarcomere. It serves as a scaffold to which myofibrils and other muscle related proteins are attached. This gene encodes a protein found in striated and cardiac muscle that binds to the titin Z1-Z2 domains and is a substrate of titin kinase, interactions thought to be critical to sarcomere assembly. Mutations in this gene are associated with limb-girdle muscular dystrophy type 2G. [provided by RefSeq, Jul 2008]
UniProt Comments for TCAP
Muscle assembly regulating factor. Mediates the antiparallel assembly of titin (TTN) molecules at the sarcomeric Z-disk.
Research Articles on TCAP
1. based on the crystal structure in which Z1Z2 and telethonin1-90 assemble into a 2:1 complex, a single chain fusion protein was designed, comprising two Z1Z2 modules that are connected by flexible linkers N- and C-terminally of the telethonin1-90. Expression of this fusion protein, named ZTZ, affords high yields of soluble expressed and purified protein
Precautions
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Disclaimer
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