Adducin, alpha, Polyclonal Antibody

Anti -Adducin, alpha, phosphorylated (Ser726) (Alpha-adducin, Erythrocyte Adducin Subunit alpha, ADD1, ADDA)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 4; NC_000004.11 (2845584..2931789). Location: 4p16.3

Polyclonal

IgG

Rabbit

Recognizes human ADD1 when phosphorylated ar (Ser726). Species Cross-Reactivity: mouse, rat.

Affinity Purified
Purified by immunoaffinity chromatography.

Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Small volumes of anti-ADD1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Adducin is a membrane skeletal protein that binds to actin filaments (F-actin) and promotes the association of spectrin with F-actin to form a spectrin-actin meshwork beneath plasma membranes. Adducin is a heterodimeric protein that consists of related subunits; alpha and beta, or alpha and gamma subunits. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinase C (PKC), and protein kinase A (PKA). Phosphorylation of adducing alpha by Rho-kinase at Thr445 and Thr480 enhances the F-actin-binding activity of adducing alpha.

Antibodies; Abs to Actin Related Proteins

Western Blot (WB), Immunohistochemistry (IHC)

Suitable for use in Western Blot and Immunohistochemistry.
Dilution: Western Blot:1:500-1:1,000
Immunohistochemistry:1:50-1:100

80,955 Da[Similar Products]

adducin 1 (alpha)

alpha-adducin; OTTHUMP00000151163; OTTHUMP00000151164; OTTHUMP00000151165; OTTHUMP00000218664; OTTHUMP00000218665; erythrocyte adducin alpha subunit; erythrocyte adducin subunit alpha

Alpha-adducin

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ADDA_HUMAN

Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described. [provided by RefSeq]

ADD1: a cytoskeletal protein that promotes the assembly of the spectrin-actin network. Adducin is a heterodimeric protein that consists of related subunits. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Three alternatively-spliced isoforms have been described.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 4p16.3

Cellular Component: nucleoplasm; F-actin capping protein complex; intermediate filament cytoskeleton; focal adhesion; perinuclear region of cytoplasm; postsynaptic density; plasma membrane; dendritic spine; nucleus; cytosol

Molecular Function: actin filament binding; calmodulin binding; protein homodimerization activity; spectrin binding; protein heterodimerization activity; T cell receptor binding; actin binding; structural molecule activity; transcription factor binding

Biological Process: actin filament bundle formation; positive regulation of protein binding; hemoglobin metabolic process; unfolded protein response; apoptosis; in utero embryonic development; multicellular organism growth; cell morphogenesis; positive regulation of endocytosis; positive regulation of angiogenesis; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; homeostasis of number of cells within a tissue; erythrocyte differentiation; barbed-end actin filament capping; cell volume homeostasis; actin cytoskeleton organization and biogenesis; cell structure disassembly during apoptosis

Disease: Hypertension, Essential

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