NAD (P) dependent steroid dehydrogenase-like, ELISA Kit

Mouse Sterol-4-alpha-carboxylate 3-dehydrogenase; decarboxylating (NSDHL) ELISA kit; H105E3; SDR31E1; XAP104; short chain dehydrogenase/reductase family 31E; member 1; sterol-4-alpha-carboxylate 3-dehydrogenase; decarboxylating; NAD (P) dependent steroid dehydrogenase-like

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of NSDHL elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9319673 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the NAD (P) dependent steroid dehydrogenase-like (NSDHL) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NSDHL. The ELISA analytical biochemical technique of the MBS9319673 kit is based on NSDHL antibody-NSDHL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NSDHL antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NSDHL. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

40,686 Da

NAD(P) dependent steroid dehydrogenase-like

sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating; striated; bare patches; NAD(P)-dependent steroid dehydrogenase-like protein

Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating

NSDHL_MOUSE

NSDHL: Defects in NSDHL are the cause of congenital hemidysplasia with ichthyosiform erythroderma and limb defects (CHILD). CHILD is an X-linked dominant disorder of lipid metabolism with disturbed cholesterol biosynthesis, which typically results in male lethality. Clinically, it is characterized by congenital, unilateral, ichthyosisform erythroderma with striking lateralization, sharp midline demarcation, and ipsilateral limb defects and hypoplasia of the body. Limbs defects range from hypoplasia of digits or ribs to complete amelia, often including scoliosis. Defects in NSDHL are the cause of CK syndrome (CKS). CKS is a disorder characterized by mild to severe cognitive impairment, seizures, microcephaly, cerebral cortical malformations, dysmorphic facial features, and thin body habitus. Belongs to the 3-beta-HSD family.

Protein type: EC 1.1.1.170; Oxidoreductase; Endoplasmic reticulum; Membrane protein, integral; Lipid Metabolism - steroid biosynthesis

Cellular Component: membrane; intracellular membrane-bound organelle; endoplasmic reticulum; integral to membrane; lipid particle

Molecular Function: 3-beta-hydroxy-delta5-steroid dehydrogenase activity; oxidoreductase activity; sterol-4-alpha-carboxylate 3-dehydrogenase (decarboxylating) activity; oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor

Biological Process: steroid metabolic process; cholesterol metabolic process; smoothened signaling pathway; hair follicle development; sterol biosynthetic process; lipid metabolic process; cholesterol biosynthetic process; steroid biosynthetic process

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