NSDHL, Recombinant Protein

Sterol-4-alpha-carboxylate 3-dehydrogenase decarboxylating; Sterol-4-alpha-carboxylate 3-dehydrogenase; decarboxylating; H105E3; SDR31E1; XAP104

For Research Use Only. Not for use in diagnostic procedures.

E Coli

> 80% by SDS-PAGE

Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol 0.1M NaCl

0.25 mg/ml (determined by Bradford assay) (lot specific)

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Small volumes of NSDHL recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

NSDHL, as known as Sterol-4-alpha-carboxylate 3-dehydrogenase, is involved in the production (synthesis) of cholesterol. During cholesterol synthesis, it participates in one of several steps that convert a molecule called lanosterol to cholesterol. Specifically, this enzyme removes a carbon atom and three hydrogen atoms (a methyl group) in the conversion of lanosterol to cholesterol. It is also an important component of cell membranes and myelin, the fatty covering that insulates nerve cells. Recombinant human NSDHL protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.

Metabolism

SDS-PAGE

35.5kDa (320aa), confirmed by MALDI-TOF

NAD(P) dependent steroid dehydrogenase-like

sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating

Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating

H105E3  [Similar Products]

The protein encoded by this gene is localized in the endoplasmic reticulum and is involved in cholesterol biosynthesis. Mutations in this gene are associated with CHILD syndrome, which is a X-linked dominant disorder of lipid metabolism with disturbed cholesterol biosynthesis, and typically lethal in males. Alternatively spliced transcript variants with differing 5' UTR have been found for this gene. [provided by RefSeq, Jul 2008]

Involved in the sequential removal of two C-4 methyl groups in post-squalene cholesterol biosynthesis.

Morimoto M. et al. (2012) J Mol Histol. 43:95-106; McLarren KW. et al. (2010) Am J Hum Genet 87:905-914.;

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