Product Name
Laforin (EPM2A), Polyclonal Antibody
Full Product Name
Anti-Laforin Antibody
Product Synonym Names
Laforin
Product Gene Name
anti-EPM2A antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O95278
Species Reactivity
Human, Mouse, Rat
Specificity
Recognizes endogenous levels of Laforin protein.
Purity/Purification
Purified by immunogen affinity chromatography
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Immunogen
Recombinant full length protein of human Laforin
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Other Notes
Small volumes of anti-EPM2A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-EPM2A antibody
Rabbit polyclonal antibody to Laforin
Applications Tested/Suitable for anti-EPM2A antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes for anti-EPM2A antibody
WB: 1/500 - 1/2000
IF/ICC: 1/50 - 1/200
Western Blot (WB) of anti-EPM2A antibody
Western blot analysis of Laforin expression in Hela (A), mouse brain (B), rat brain (C) whole cell lysates.
Immunofluorescence (IF) of anti-EPM2A antibody
Immunofluorescent analysis of Laforin staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
NCBI/Uniprot data below describe general gene information for EPM2A. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001018051.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001018041.1
[Other Products]
UniProt Primary Accession #
O95278
[Other Products]
UniProt Secondary Accession #
O95483; Q5T3F5; Q6IS15; Q8IU96; Q8IX24; Q8IX25; Q9BS66; Q9UEN2; B3KMU5; B4DRZ2[Other Products]
UniProt Related Accession #
O95278; B3EWF7[Other Products]
Molecular Weight
35,169 Da
NCBI Official Full Name
laforin isoform b
NCBI Official Synonym Full Names
EPM2A, laforin glucan phosphatase
NCBI Official Symbol
EPM2A [Similar Products]
NCBI Official Synonym Symbols
EPM2; MELF
[Similar Products]
NCBI Protein Information
laforin
UniProt Protein Name
Laforin
UniProt Synonym Protein Names
Glucan phosphatase
UniProt Gene Name
EPM2A [Similar Products]
UniProt Synonym Gene Names
LAFPTPase [Similar Products]
NCBI Summary for EPM2A
This gene encodes a dual-specificity phosphatase that associates with polyribosomes. The encoded protein may be involved in the regulation of glycogen metabolism. Mutations in this gene have been associated with myoclonic epilepsy of Lafora. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]
UniProt Comments for EPM2A
Plays an important role in preventing glycogen hyperphosphorylation and the formation of insoluble aggregates, via its activity as glycogen phosphatase, and by promoting the ubiquitination of proteins involved in glycogen metabolism via its interaction with the E3 ubiquitin ligase NHLRC1/malin. Shows strong phosphatase activity towards complex carbohydrates in vitro, avoiding glycogen hyperphosphorylation which is associated with reduced branching and formation of insoluble aggregates (PubMed:16901901, PubMed:23922729, PubMed:26231210, PubMed:25538239, PubMed:25544560). Dephosphorylates phosphotyrosine and synthetic substrates, such as para-nitrophenylphosphate (pNPP), and has low activity with phosphoserine and phosphothreonine substrates (in vitro) (PubMed:11001928, PubMed:11220751, PubMed:11739371, PubMed:14532330, PubMed:16971387, PubMed:18617530, PubMed:22036712, PubMed:23922729, PubMed:14722920). Has been shown to dephosphorylate MAPT (). Forms a complex with NHLRC1/malin and HSP70, which suppresses the cellular toxicity of misfolded proteins by promoting their degradation through the ubiquitin-proteasome system (UPS). Acts as a scaffold protein to facilitate PPP1R3C/PTG ubiquitination by NHLRC1/malin (PubMed:23922729). Also promotes proteasome-independent protein degradation through the macroautophagy pathway (PubMed:20453062).
Research Articles on EPM2A
1. rs702304 and rs2235481 within the EPM2A gene were associated with schizophrenia liability.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
