GNAS, Polyclonal Antibody

Anti-GNAS Antibody IHC-plus; GNAS; AHO; C20orf45; Extra large alphas protein; GSP; GNAS complex locus; GNASXL; NESP; PHP1B; PHP1C; PHP1A; POH; SCG6; XLalphas; GNAS1; GPSA; GSA; NESP55; Protein ALEX; Secretogranin VI; Human GNAS

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Human GNAS. Predicted cross-reactivity based on amino acid sequence homology: mouse (88%), rat (100%), bovine (100%), pig (90%).

Immunoaffinity Purified

0.1 M Tris-glycine, pH 7, 10% Glycerol, 0.01% Thimerosal

1 mg/ml (lot specific)

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Small volumes of anti-GNAS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

The GNAS1 gene encodes the alpha subunit of the G protein Gs, which couples receptor binding by several hormones to activation of adenylate cyclase (Hayward et al., 1998). This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons.

Immunohistochemistry (IHC - Paraffin), Western Blot (WB)

IHC-P (5 ug/ml), WB (1:500 - 1:3000)

Anti-GNAS antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Anti-GNAS antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Sample (30 ug of whole cell lysate). A: Hep G2. B: Raji. 10% SDS PAGE. GNAS antibody diluted at 1:1000.

45,665 Da

GNAS complex locus

protein ALEX; protein GNAS; protein NESP55; secretogranin VI; extra large alphas protein; neuroendocrine secretory protein; guanine nucleotide regulatory protein; alternative gene product encoded by XL-exon; adenylate cyclase-stimulating G alpha protein; guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas; guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1

Guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas

; XLalphas  [Similar Products]

GNAS1_HUMAN

This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contain a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. An antisense transcript is produced from an overlapping locus on the opposite strand. One of the transcripts produced from this locus, and the antisense transcript, are paternally expressed noncoding RNAs, and may regulate imprinting in this region. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors. [provided by RefSeq, Aug 2012]

G-alpha(s) iso4: a guanine nucleotide-binding protein of the G12 class of G-alpha proteins. Agonist binding to Gq-coupled receptors may block Akt activation via the release of active G-alpha(q) subunits that inhibit phosphatidylinositol 3-kinase. Heterotrimeric G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide .

Protein type: G protein, heterotrimeric; Membrane protein, peripheral; G protein, heterotrimeric alpha G(s); Vesicle; Oncoprotein; G protein

Chromosomal Location of Human Ortholog: 20q13.3

Cellular Component: ruffle; transport vesicle; membrane; perinuclear region of cytoplasm; cytoplasm; dendrite; extracellular region; plasma membrane; heterotrimeric G-protein complex; intrinsic to membrane; trans-Golgi network membrane; nucleus; cytosol

Molecular Function: corticotropin-releasing hormone receptor 1 binding; GTPase activity; GTP binding; metal ion binding; adenylate cyclase activity; ionotropic glutamate receptor binding; insulin-like growth factor receptor binding; signal transducer activity; protein binding; mu-type opioid receptor binding; G-protein beta/gamma-subunit binding; beta-2 adrenergic receptor binding; D1 dopamine receptor binding

Biological Process: developmental growth; positive regulation of osteoclast differentiation; water transport; protein secretion; female pregnancy; G-protein signaling, adenylate cyclase activating pathway; embryonic hindlimb morphogenesis; intracellular transport; positive regulation of cAMP biosynthetic process; DNA methylation; tissue homeostasis; transmembrane transport; post-embryonic body morphogenesis; dopamine receptor, adenylate cyclase activating pathway; endochondral ossification; response to drug; sensory perception of chemical stimulus; negative regulation of multicellular organism growth; multicellular organism growth; adenylate cyclase activation; sensory perception of smell; embryonic cranial skeleton morphogenesis; positive regulation of osteoblast differentiation; cartilage development; energy reserve metabolic process; renal water homeostasis; cAMP biosynthetic process; blood coagulation; cognition; regulation of insulin secretion

Disease: Acth-independent Macronodular Adrenal Hyperplasia; Pseudohypoparathyroidism, Type Ib

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