FOXC1, ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

This kit recognizes natural and recombinant Human FOXC1. No significant cross-reactivity or interference between Human FOXC1 and analogues was observed.

Store at 4 degree C.

Manufactured in an ISO 9001:2015 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of FOXC1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2509968 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the FOXC1, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing FOXC1. The ELISA analytical biochemical technique of the MBS2509968 kit is based on FOXC1 antibody-FOXC1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FOXC1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FOXC1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to FOXC1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for FOXC1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain FOXC1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of FOXC1. You can calculate the concentration of FOXC1 in the samples by comparing the OD of the samples to the standard curve.

56,789 Da

forkhead box C1

forkhead box protein C1; forkhead box C1 protein; forkhead, drosophila, homolog-like 7; forkhead-related activator 3; forkhead-related protein FKHL7; forkhead-related transcription factor 3; forkhead/winged helix-like transcription factor 7; myeloid factor-delta

Forkhead box protein C1

FKHL7; FREAC3; FREAC-3  [Similar Products]

FOXC1_HUMAN

This gene belongs to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of this gene has not yet been determined; however, it has been shown to play a role in the regulation of embryonic and ocular development. Mutations in this gene cause various glaucoma phenotypes including primary congenital glaucoma, autosomal dominant iridogoniodysgenesis anomaly, and Axenfeld-Rieger anomaly. [provided by RefSeq, Jul 2008]

FOXC1: Binding of FREAC-3 and FREAC-4 to their cognate sites results in bending of the DNA at an angle of 80-90 degrees. Monomer. Expressed in all tissues and cell lines examined.

Protein type: Cell development/differentiation; Transcription factor; Cell cycle regulation; DNA-binding

Chromosomal Location of Human Ortholog: 6p25

Cellular Component: nucleoplasm; cytoplasm; nuclear heterochromatin; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; DNA binding; sequence-specific DNA binding; transcription factor activity; DNA bending activity; transcription factor binding

Biological Process: transcription from RNA polymerase II promoter; collagen fibril organization; neural crest cell development; glycosaminoglycan metabolic process; paraxial mesoderm formation; heart development; positive regulation of transcription, DNA-dependent; cardiac muscle cell proliferation; regulation of transcription, DNA-dependent; ovarian follicle development; ureteric bud development; germ cell migration; ventricular cardiac muscle morphogenesis; negative regulation of mitotic cell cycle; lacrimal gland development; regulation of blood vessel size; skeletal development; mesenchymal cell development; somitogenesis; ossification; cell migration; Notch signaling pathway; camera-type eye development; in utero embryonic development; regulation of organ growth; odontogenesis of dentine-containing teeth; cell proliferation; eye development; artery morphogenesis; embryonic heart tube development; blood vessel remodeling; positive regulation of transcription from RNA polymerase II promoter; brain development; vascular endothelial growth factor receptor signaling pathway; lymph vessel development

Disease: Axenfeld-rieger Syndrome, Type 3; Iridogoniodysgenesis, Type 1

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