GNAS, Polyclonal Antibody

AHO, GSA, GSP, POH, GPSA, NESP, GNAS1, PHP1A, PHP1B, PHP1C, C20orf45

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

Antigen affinity purification

1mg/mL (lot specific)

Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Manufactured in an ISO 9001:2015 Certified Laboratory.

Small volumes of anti-GNAS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contain a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. An antisense transcript is produced from an overlapping locus on the opposite strand. One of the transcripts produced from this locus, and the antisense transcript, are paternally expressed noncoding RNAs, and may regulate imprinting in this region. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors.

Cancer; Epigenetics

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

WB: 1:200-1:1000
IHC: 1:25-1:100

Western Blot analysis of Mouse brain tissue using GNAS Polyclonal Antibody at dilution of 1:500

Immunohistochemistry of paraffin-embedded Human liver cancer using GNAS Polyclonal Antibody at dilution of 1:30

45,664 Da

GNAS complex locus

ALEX; GNAS; NESP55; G-alpha-8; GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus; adenylate cyclase-stimulating G alpha protein; gnas {ECO:0000312|RGD:2716}; guanine nucleotide binding protein alpha s; guanine nucleotide-binding protein G-s, alpha subunit

Guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas

XLalphas  [Similar Products]

GNAS1_RAT

This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contain a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants have been found for this gene. [provided by RefSeq, Apr 2009]

G-alpha(s): a guanine nucleotide-binding protein of the G12 class of G-alpha proteins. Agonist binding to Gq-coupled receptors may block Akt activation via the release of active G-alpha(q) subunits that inhibit phosphatidylinositol 3-kinase. Heterotrimeric G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide .

Protein type: G protein, heterotrimeric alpha G(s); G protein, heterotrimeric; G protein; Oncoprotein; Vesicle; Membrane protein, peripheral

Cellular Component: dendrite; extracellular region; cytosol; lipid raft; ruffle; synaptic vesicle; membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; heterotrimeric G-protein complex; intrinsic to membrane; trans-Golgi network membrane; nucleus; cell junction; sarcolemma; vesicle; endosome

Molecular Function: corticotropin-releasing hormone receptor 1 binding; GTPase activity; protein domain specific binding; GTP binding; metal ion binding; G-protein alpha-subunit binding; G-protein beta-subunit binding; ionotropic glutamate receptor binding; insulin-like growth factor receptor binding; signal transducer activity; protein binding; mu-type opioid receptor binding; G-protein beta/gamma-subunit binding; beta-2 adrenergic receptor binding; D1 dopamine receptor binding; alpha-tubulin binding

Biological Process: skin development; developmental growth; metabolic process; positive regulation of osteoclast differentiation; G-protein signaling, adenylate cyclase activating pathway; embryonic hindlimb morphogenesis; post-embryonic development; calcium ion homeostasis; positive regulation of cAMP biosynthetic process; DNA methylation; tissue homeostasis; negative regulation of blood pressure; skeletal development; endochondral ossification; dopamine receptor, adenylate cyclase activating pathway; post-embryonic body morphogenesis; response to drug; sensory perception of chemical stimulus; negative regulation of multicellular organism growth; multicellular organism growth; embryonic cranial skeleton morphogenesis; regulation of transcription from RNA polymerase II promoter; positive regulation of osteoblast differentiation; G-protein coupled receptor protein signaling pathway; cartilage development; energy reserve metabolic process; positive regulation of protein amino acid phosphorylation; cognition

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