Full Product Name
AIRE Antibody
Product Synonym Names
AIRE1; APECED; APS1; APSI; autoimmune polyendocrinopathy candidiasis ectodermal dystrophy; PGA1
Product Gene Name
anti-AIRE antibody
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O43918
Specificity
AIRE Antibody detects endogenous levels of total AIRE protein.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (lot specific)
Target Modification
Unmodified/Total
Immunogen
The antiserum was produced against synthesized peptide derived from human AIRE.
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Other Notes
Small volumes of anti-AIRE antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-AIRE antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-AIRE antibody
WB: 1:500~1:1000
ELISA: 1:5000
Western Blot (WB) of anti-AIRE antibody
Western blot analysis of lysates from HeLa cells, treated with Hu 2nM 24h, using AIRE Antibody. The lane on the right is blocked with the synthesized peptide.
NCBI/Uniprot data below describe general gene information for AIRE. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000374.1
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NCBI GenBank Nucleotide #
NM_000383.3
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UniProt Primary Accession #
O43918
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UniProt Secondary Accession #
O43922; O43932; O75745; B2RP50[Other Products]
UniProt Related Accession #
O43918[Other Products]
NCBI Official Full Name
autoimmune regulator
NCBI Official Synonym Full Names
autoimmune regulator
NCBI Official Symbol
AIRE [Similar Products]
NCBI Official Synonym Symbols
APS1; APSI; PGA1; AIRE1; APECED
[Similar Products]
NCBI Protein Information
autoimmune regulator
UniProt Protein Name
Autoimmune regulator
UniProt Synonym Protein Names
Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein; APECED protein
Protein Family
Autoimmune regulator
UniProt Gene Name
AIRE [Similar Products]
UniProt Synonym Gene Names
APECED; APECED protein [Similar Products]
NCBI Summary for AIRE
This gene encodes a transcriptional regulator that forms nuclear bodies and interacts with the transcriptional coactivator CREB binding protein. The encoded protein plays an important role in immunity by regulating the expression of autoantigens and negative selection of autoreactive T-cells in the thymus. Mutations in this gene cause the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy with candidiasis and ectodermal dystrophy (APECED). [provided by RefSeq, Jun 2012]
UniProt Comments for AIRE
Transcription factor playing an essential role to promote self-tolerance in the thymus by regulating the expression of a wide array of self-antigens that have the commonality of being tissue-restricted in their expression pattern in the periphery, called tissue restricted antigens (TRA) (PubMed:26084028). Binds to G-doublets in an A/T-rich environment; the preferred motif is a tandem repeat of 5'-ATTGGTTA-3' combined with a 5'-TTATTA-3' box. Binds to nucleosomes (). Binds to chromatin and interacts selectively with histone H3 that is not methylated at 'Lys-4', not phosphorylated at 'Thr-3' and not methylated at 'Arg-2'. Functions as a sensor of histone H3 modifications that are important for the epigenetic regulation of gene expression. Mainly expressed by medullary thymic epithelial cells (mTECs), induces the expression of thousands of tissue-restricted proteins, which are presented on major histocompatibility complex class I (MHC-I) and MHC-II molecules to developing T-cells percolating through the thymic medulla (PubMed:26084028). Also induces self-tolerance through other mechanisms such as the regulation of the mTEC differentiation program. Controls the medullary accumulation of thymic dendritic cells and the development of regulatory T-cell through the regulation of XCL1 expression. Regulates the production of CCR4 and CCR7 ligands in medullary thymic epithelial cells and alters the coordinated maturation and migration of thymocytes. In thimic B-cells, allows the presentation of licensing-dependent endogenous self-anitgen for negative selection. In secondary lymphoid organs, induces functional inactivation of CD4+ T-cells. Expressed by a distinct bone marrow-derived population, induces self-tolerance through a mechanism that does not require regulatory T-cells and is resitant to innate inflammatory stimuli ().
Research Articles on AIRE
1. Rs3761389 variant is associated with the susceptibility of myasthenia gravis in Chinese patients.
Precautions
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