Full Product Name
VSX1 siRNA (Human)
Product Synonym Names
RINX; Visual system homeobox 1; Homeodomain protein RINX; Retinal inner nuclear layer homeobox protein; Transcription factor VSX1
Product Gene Name
VSX1 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9NZR4
Specificity
VSX1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human VSX1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of VSX1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
VSX1 sirna
siRNA to inhibit VSX1 expression using RNA interference
Applications Tested/Suitable for VSX1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for VSX1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001243200.1
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NCBI GenBank Nucleotide #
NM_001256271.1
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UniProt Primary Accession #
Q9NZR4
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UniProt Secondary Accession #
Q0GM60; Q0GM61; Q0GM62; Q0GM63; Q0GM64; Q0GM65; Q5TF40; Q5TF41; Q9HCU3; B9EGJ4; D1MF28[Other Products]
UniProt Related Accession #
Q9NZR4[Other Products]
Molecular Weight
32,054 Da
NCBI Official Full Name
visual system homeobox 1 isoform c
NCBI Official Synonym Full Names
visual system homeobox 1
NCBI Official Symbol
VSX1 [Similar Products]
NCBI Official Synonym Symbols
PPD; KTCN; PPCD; RINX; KTCN1; PPCD1; CAASDS
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NCBI Protein Information
visual system homeobox 1
UniProt Protein Name
Visual system homeobox 1
UniProt Synonym Protein Names
Homeodomain protein RINX; Retinal inner nuclear layer homeobox protein; Transcription factor VSX1
Protein Family
Visual system homeobox
UniProt Gene Name
VSX1 [Similar Products]
UniProt Synonym Gene Names
RINX [Similar Products]
UniProt Entry Name
VSX1_HUMAN
NCBI Summary for VSX1
The protein encoded by this gene contains a paired-like homeodomain and binds to the core of the locus control region of the red/green visual pigment gene cluster. The encoded protein may regulate expression of the cone opsin genes early in development. Mutations in this gene can cause posterior polymorphous corneal dystrophy and keratoconus. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
UniProt Comments for VSX1
VSX1: Binds to the 37-bp core of the locus control region (LCR) of the red/green visual pigment gene cluster. May regulate the activity of the LCR and the cone opsin genes at earlier stages of development. Defects in VSX1 are a cause of posterior polymorphous corneal dystrophy type 1 (PPCD1). PPCD1 is a slowly progressive hereditary disorder of the corneal endothelium that leads to a variable degree of visual impairment usually in *****hood. Defects in VSX1 are a cause of keratoconus type 1 (KTCN1). Keratoconus type 1 is a frequent corneal dystrophy with an incidence that varies from 50 to 230 per 100'000. The cornea assumes a conical shape as a result of a progressive non- inflammatory thinning of the corneal stroma. Keratoconus is most often an isolated sporadic condition with cases of autosomal dominant and autosomal recessive transmission. Defects in VSX1 are the cause of craniofacial anomalies and anterior segment dysgenesis syndrome (CAASDS). CAASDS is a disorder with extremely variable expressivity. Clinical features include wide interpupillary distance, abnormal corneal endothelium, unusual pinnae, partially to completely empty sella turcica, posterior fossa cyst, anterior encephalocele, and/or hydrocephalus. Belongs to the paired homeobox family. 8 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding; Cell development/differentiation
Chromosomal Location of Human Ortholog: 20p11.21
Cellular Component: nucleus
Molecular Function: sequence-specific DNA binding; transcription factor activity
Biological Process: regulation of transcription, DNA-dependent; visual perception; transcription, DNA-dependent; response to stimulus; neuron maturation; retinal bipolar neuron differentiation
Disease: Corneal Dystrophy, Posterior Polymorphous, 1; Keratoconus 1; Craniofacial Anomalies And Anterior Segment Dysgenesis Syndrome
Research Articles on VSX1
1. novel missense substitutions in the VSX1 gene: clinical and genetic analysis of families with Keratoconus from India
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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