1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1, ELISA Kit

1-phosphatidylinositol-4; 5-bisphosphate phosphodiesterase gamma-1; PLC-148; Phosphoinositide phospholipase C-gamma-1; Phospholipase C-II; PLC-II; Phospholipase C-gamma-1

For Research Use Only. Not for use in diagnostic procedures.

This assay recognizes recombinant and natural human 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1. No significant cross-reactivity or interference was observed.

For long term storage, please store the entire kit at -20 degree C.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of PLCG1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2885880 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 (PLCG1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PLCG1. The ELISA analytical biochemical technique of the MBS2885880 kit is based on PLCG1 antibody-PLCG1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PLCG1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PLCG1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of human 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

148,660 Da

phospholipase C gamma 1

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1

PLC1; PLC-II; PLC-gamma-1  [Similar Products]

PLCG1_HUMAN

The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

PLCG1: a calcium dependent phosphatidylinositol-specific phospholipase C. The activated enzyme produces the second messenger molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylated and activated by tyrosine kinases in response to signaling through a variety of growth factor receptors and immune system receptors.

Protein type: EC 3.1.4.11; Phospholipase; Carbohydrate Metabolism - inositol phosphate; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 20q12-q13.1

Cellular Component: cell projection; cytoplasm; cytosol; intercellular junction; lamellipodium; plasma membrane; ruffle; signalosome

Molecular Function: calcium ion binding; glutamate receptor binding; neurotrophin TRKA receptor binding; phosphoinositide phospholipase C activity; phospholipase C activity; protein binding; protein kinase binding; receptor signaling protein activity; receptor tyrosine kinase binding

Biological Process: activation of MAPKK activity; axon guidance; blood coagulation; calcium-mediated signaling; cell migration; cytokine and chemokine mediated signaling pathway; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; in utero embryonic development; innate immune response; inositol phosphate metabolic process; leukocyte migration; nerve growth factor receptor signaling pathway; phospholipase C activation; phospholipid catabolic process; positive regulation of angiogenesis; positive regulation of blood vessel endothelial cell migration; positive regulation of release of sequestered calcium ion into cytosol; signal transduction; T cell receptor signaling pathway; vascular endothelial growth factor receptor signaling pathway; viral reproduction

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