ATP-dependent DNA helicase Q4 (RECQL4), ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of RECQL4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7237745 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ATP-dependent DNA helicase Q4 (RECQL4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing RECQL4. The ELISA analytical biochemical technique of the MBS7237745 kit is based on RECQL4 antibody-RECQL4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RECQL4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RECQL4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Epigenetics and Nuclear Signaling

133,077 Da

RecQ protein-like 4

ATP-dependent DNA helicase Q4; DNA helicase, RecQ-like type 4; DNA helicase, RecQ-like, type 4

ATP-dependent DNA helicase Q4

RECQ4; RecQ4  [Similar Products]

RECQ4_HUMAN

The protein encoded by this gene is a DNA helicase that belongs to the RecQ helicase family. DNA helicases unwind double-stranded DNA into single-stranded DNAs and may modulate chromosome segregation. This gene is predominantly expressed in thymus and testis. Mutations in this gene are associated with Rothmund-Thomson, RAPADILINO and Baller-Gerold syndromes. [provided by RefSeq, Jan 2010]

RECQL4: DNA-dependent ATPase. May modulate chromosome segregation. Interacts with UBR1 and UBR2. Interacts with MCM10; this interaction regulates RECQL4 unwinding activity. Up-regulated in actively dividing cells. Ubiquitously expressed, with highest levels in thymus and testis. Belongs to the helicase family. RecQ subfamily.

Protein type: Helicase; EC 3.6.4.12

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: membrane; cytoplasm; nucleus

Molecular Function: ATP-dependent 3'-5' DNA helicase activity; zinc ion binding; bubble DNA binding; ATP binding

Biological Process: multicellular organismal development; DNA strand renaturation; DNA repair; DNA replication; DNA duplex unwinding; DNA recombination

Disease: Rapadilino Syndrome; Rothmund-thomson Syndrome; Baller-gerold Syndrome

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