14-3-3 gamma, Polyclonal Antibody

14-3-3 g Antibody; 1433G; 143G; KCIP-1; Protein kinase C inhibitor protein-1; YWHAG

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

The antibody detects endogenous levels of total 14-3-3 g protein.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

1.0 mg/ml (lot specific)

Store at -20 degree C

Small volumes of anti-14-3-3 gamma antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.Autieri M.V., DNA Cell Biol. 18:555-564(1999).Horie M., Genomics 60:241-243(1999).Hillier L.W., Nature 424:157-164(2003).

Total protein Ab

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500

Western blot analysis of extracts from K562 cells, treated with insulin (0.01U/ml, 15mins), using 14-3-3 g antibody.

Immunohistochemistry analysis of paraffin-embedded human brain tissue using 14-3-3 g antibody.

Immunofluorescence analysis of COS7 cells, using 14-3-3 g antibody.

28,303 Da

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma

14-3-3 protein gamma

14-3-3 protein gamma

KCIP-1  [Similar Products]

1433G_HUMAN

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. [provided by RefSeq, Jul 2008]

14-3-3 gamma: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Homodimer. Interacts with SAMSN1. Interacts with RAF1, SSH1 and CRTC2/TORC2. Interacts with ABL1 (phosphorylated form); the interaction retains it in the cytoplasm. Interacts with GAB2. Interacts with MDM4 (phosphorylated); negatively regulates MDM4 activity toward TP53. Interacts with PKA-phosphorylated AANAT. Highly expressed in brain, skeletal muscle, and heart. Belongs to the 14-3-3 family.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: cytoplasmic vesicle membrane; focal adhesion; membrane; cytosol

Molecular Function: protein kinase C inhibitor activity; protein domain specific binding; insulin-like growth factor receptor binding; protein binding; protein kinase C binding; receptor tyrosine kinase binding

Biological Process: cellular response to insulin stimulus; apoptosis; regulation of neuron differentiation; organelle organization and biogenesis; regulation of signal transduction; negative regulation of protein kinase activity; regulation of synaptic plasticity; mitotic cell cycle; G2/M transition of mitotic cell cycle; protein targeting

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