Product Name
Tropomyosin alpha (TMSA), Polyclonal Antibody
Full Product Name
Tropomyosin alpha Antibody
Product Gene Name
anti-TMSA antibody
[Similar Products]
Product Synonym Gene Name
TPM1[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P09493
Species Reactivity
Human, Mouse, Rat
Specificity
Tropomyosin alpha antibody detects endogenous levels of Tropomyosin alpha.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (lot specific)
Target Modification
Unmodified/Total
Immunogen
The antiserum was produced against synthesized peptide derived from human Tropomyosin alpha.
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Other Notes
Small volumes of anti-TMSA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-TMSA antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-TMSA antibody
WB: 1:500~1:1000
ELISA: 1:10000
Western Blot (WB) of anti-TMSA antibody
Western blot analysis of lysate from HeLa cells, using Tropomyosin alpha antibody.
NCBI/Uniprot data below describe general gene information for TMSA. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000357.3
[Other Products]
NCBI GenBank Nucleotide #
NM_000366.5
[Other Products]
UniProt Primary Accession #
P09493
[Other Products]
UniProt Secondary Accession #
P09494; P10469; Q6DV89; Q6DV90; Q7Z6L8; Q86W64; Q96IK2; B7Z5T7; D9YZV2; D9YZV3; D9YZV8[Other Products]
UniProt Related Accession #
P09493[Other Products]
NCBI Official Full Name
tropomyosin alpha-1 chain isoform Tpm1.5cy
NCBI Official Synonym Full Names
tropomyosin 1
NCBI Official Symbol
TPM1 [Similar Products]
NCBI Official Synonym Symbols
CMH3; TMSA; CMD1Y; LVNC9; C15orf13; HEL-S-265; HTM-alpha
[Similar Products]
NCBI Protein Information
tropomyosin alpha-1 chain
UniProt Protein Name
Tropomyosin alpha-1 chain
UniProt Synonym Protein Names
Alpha-tropomyosin; Tropomyosin-1
Protein Family
Tropomyosin
UniProt Gene Name
TPM1 [Similar Products]
UniProt Synonym Gene Names
C15orf13; TMSA [Similar Products]
NCBI Summary for TMSA
This gene is a member of the tropomyosin family of highly conserved, widely distributed actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosin is composed of two alpha-helical chains arranged as a coiled-coil. It is polymerized end to end along the two grooves of actin filaments and provides stability to the filaments. The encoded protein is one type of alpha helical chain that forms the predominant tropomyosin of striated muscle, where it also functions in association with the troponin complex to regulate the calcium-dependent interaction of actin and myosin during muscle contraction. In smooth muscle and non-muscle cells, alternatively spliced transcript variants encoding a range of isoforms have been described. Mutations in this gene are associated with type 3 familial hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]
UniProt Comments for TMSA
Binds to actin filaments in muscle and non-muscle cells (PubMed:23170982). Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction (PubMed:23170982). Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.
Research Articles on TMSA
1. Tpm isoforms 1.8/9 are enriched in the lamellipodium of fibroblasts as detected with a novel isoform-specific monoclonal antibody. RNAi-mediated silencing of Tpm1.8/9 led to an increase of Arp2/3 accumulation at the cell periphery and a decrease in the persistence of lamellipodia and cell motility.
Precautions
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