Tropomyosin alpha-1 chain (TPM1), ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of TPM1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7218973 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Tropomyosin alpha-1 chain (TPM1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TPM1. The ELISA analytical biochemical technique of the MBS7218973 kit is based on TPM1 antibody-TPM1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TPM1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TPM1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Signal Transduction

32,866 Da

tropomyosin 1 (alpha)

tropomyosin alpha-1 chain; alpha-tropomyosin; sarcomeric tropomyosin kappa; cardiomyopathy, hypertrophic 3

Tropomyosin alpha-1 chain

C15orf13; TMSA  [Similar Products]

TPM1_HUMAN

This gene is a member of the tropomyosin family of highly conserved, widely distributed actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosin is composed of two alpha-helical chains arranged as a coiled-coil. It is polymerized end to end along the two grooves of actin filaments and provides stability to the filaments. The encoded protein is one type of alpha helical chain that forms the predominant tropomyosin of striated muscle, where it also functions in association with the troponin complex to regulate the calcium-dependent interaction of actin and myosin during muscle contraction. In smooth muscle and non-muscle cells, alternatively spliced transcript variants encoding a range of isoforms have been described. Mutations in this gene are associated with type 3 familial hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]

TPM1: a cytoskeletal protein that binds to actin filaments in muscle and nonmuscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. Defects in TPM3 are a cause of nemaline myopathy type 1 (NEM1). Three alternatively spliced isoforms have been described.

Protein type: Actin-binding; Motor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 15q22.1

Cellular Component: filamentous actin; sarcomere; cytoskeleton; stress fiber; muscle thin filament tropomyosin; cytosol

Molecular Function: structural constituent of cytoskeleton; structural constituent of muscle; cytoskeletal protein binding; actin binding

Biological Process: positive regulation of cell adhesion; wound healing; regulation of muscle contraction; in utero embryonic development; cytoskeleton organization and biogenesis; sarcomere organization; regulation of heart contraction; muscle filament sliding; muscle contraction; positive regulation of ATPase activity; positive regulation of stress fiber formation; ruffle organization and biogenesis; ventricular cardiac muscle morphogenesis; cell motility; negative regulation of cell migration; positive regulation of heart rate by epinephrine; cardiac muscle contraction

Disease: Cardiomyopathy, Dilated, 1y; Cardiomyopathy, Familial Hypertrophic, 3

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