Serine/threonine-protein kinase VRK1 (Vrk1), Recombinant Protein

Serine/threonine-protein kinase VRK1; EC=2.7.11.1; Serine/threonine-protein kinase 51PK; Vaccinia-related kinase 1

For Research Use Only. Not for use in diagnostic procedures.

E Coli or Yeast or Baculovirus or Mammalian Cell

>=90% (lot specific)

Liquid containing glycerol

This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.

Sterile filter available upon request.

Low endotoxin available upon request.

Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Small volumes of Vrk1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Serine/threonine kinase involved in Golgi disassembly during the cell cycle: following phosphorylation by PLK3 during mitosis, required to induce Golgi fragmentation. Acts by mediating phosphorylation of downstream target protein. Phosphorylates 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Phosphorylates casein and histone H3. Phosphorylates BANF1: disrupts its ability to bind DNA, reduces its binding to LEM domain-containing proteins and causes its relocalization from the nucleus to the cytoplasm. Phosphorylates ATF2 which activates its transcriptional activity

Cell Biology

(Note: Representative image, actual molecular weight may vary depending on Tag type and expression host)

47,228 Da

vaccinia related kinase 1

serine/threonine-protein kinase VRK1; vaccinia-related kinase 1; serine/threonine-protein kinase 51PK

Serine/threonine-protein kinase VRK1

VRK1_MOUSE

VRK1: Serine/threonine kinase that phosphorylates Thr-18 of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Defects in VRK1 are the cause of pontocerebellar hypoplasia type 1 (PCH1); also called pontocerebellar hypoplasia with infantile spinal muscular atrophy or pontocerebellar hypoplasia with anterior horn cell disease. PCH1 is characterized by an abnormally small cerebellum and brainstem, central and peripheral motor dysfunction from birth, gliosis and anterior horn cell degeneration resembling infantile spinal muscular atrophy (SMA). Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. VRK subfamily.

Protein type: Kinase, protein; Protein kinase, CK1; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; CK1 group; VRK family

Cellular Component: nucleoplasm; Golgi stack; cytoskeleton; cytoplasm; nucleolus; nucleus

Molecular Function: histone serine kinase activity (H3-S10 specific); transferase activity; protein serine/threonine kinase activity; nucleosomal histone binding; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; protein kinase binding; ATP binding; protein kinase activity

Biological Process: regulation of cell shape; mitosis; peptidyl-serine phosphorylation; Wnt receptor signaling pathway; cell division; protein amino acid autophosphorylation; endocytosis; cell cycle; protein amino acid phosphorylation; phosphorylation

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