Product Name
Connexin 50/GJA8, Polyclonal Antibody
Full Product Name
Goat anti-Connexin 50/GJA8 Antibody
Product Synonym Names
GJA8; gap junction protein, alpha 8, 50kDa; CAE; CAE1; CX50; CZP1; MP70; OTTHUMP00000025757; connexin 50; connexin-50; gap junction alpha-8 protein; gap junction membrane channel protein alpha-8; lens fiber protein MP70; lens intrinsic membrane protein MP; GJA8 antibody; gap junction protein; alpha 8; 50kDa antibody; CAE antibody; CAE1 antibody; CX50 antibody; CZP1 antibody; MP70 antibody; OTTHUMP00000025757 antibody; connexin 50 antibody; gap junction membrane channel protein alpha-8 antibody; connexin-50 antibody; gap junction alpha-8 protein antibody; lens fiber protein MP70 antibody; lens intrinsic membrane protein MP70 antibody; Connexin 50; Connexin 50 / GJA8
Product Gene Name
anti-GJA8 antibody
[Similar Products]
Antibody/Peptide Pairs
Connexin 50 / GJA8 peptide (MBS427188) is used for blocking the activity of Connexin 50/GJA8 antibody (MBS422269)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
TPSLCPELTT DDAR
Species Reactivity
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence TPSLCPELTTDDAR, from the internal region of the protein sequence according to NP_005258.2.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-GJA8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-GJA8 antibody
Peptide ELISA (EIA)
Application Notes for anti-GJA8 antibody
Peptide ELISA: Antibody detection limit dilution 1: 4000.
Western Blot: Preliminary experiments in Human Brain (Spinal Cord), Mouse and Rat Eye lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml).
NCBI/Uniprot data below describe general gene information for GJA8. It may not necessarily be applicable to this product.
NCBI Accession #
NP_005258.2
[Other Products]
NCBI GenBank Nucleotide #
NM_005267.4
[Other Products]
UniProt Secondary Accession #
Q5VVN9; Q9NP25; A7L5M5[Other Products]
UniProt Related Accession #
P48165[Other Products]
Molecular Weight
48,229 Da
NCBI Official Full Name
gap junction alpha-8 protein
NCBI Official Synonym Full Names
gap junction protein alpha 8
NCBI Official Symbol
GJA8 [Similar Products]
NCBI Official Synonym Symbols
CAE; CAE1; CX50; CZP1; MP70; CTRCT1
[Similar Products]
NCBI Protein Information
gap junction alpha-8 protein
UniProt Protein Name
Gap junction alpha-8 protein
UniProt Synonym Protein Names
Connexin-50; Cx50; Lens fiber protein MP70
Protein Family
Gap junction alpha-8 protein
UniProt Gene Name
GJA8 [Similar Products]
UniProt Synonym Gene Names
Cx50 [Similar Products]
UniProt Entry Name
CXA8_HUMAN
NCBI Summary for GJA8
This gene encodes a transmembrane connexin protein that is necessary for lens growth and maturation of lens fiber cells. The encoded protein is a component of gap junction channels and functions in a calcium and pH-dependent manner. Mutations in this gene have been associated with zonular pulverulent cataracts, nuclear progressive cataracts, and cataract-microcornea syndrome. [provided by RefSeq, Dec 2009]
UniProt Comments for GJA8
GJA8: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. A connexon is composed of a hexamer of connexins. This particular connexin only forms junctional channels. Eye lens. Belongs to the connexin family. Alpha-type (group II) subfamily.
Protein type: Channel, misc.; Membrane protein, multi-pass; Membrane protein, integral
Chromosomal Location of Human Ortholog: 1q21.1
Cellular Component: connexon complex; integral to plasma membrane
Molecular Function: channel activity; gap junction channel activity
Biological Process: cell-cell signaling; lens development in camera-type eye; protein homooligomerization; transmembrane transport; transport; visual perception
Disease: Cataract 1, Multiple Types; Chromosome 1q21.1 Deletion Syndrome, 1.35-mb
Research Articles on GJA8
1. These results indicated that the mutant Cx50 (S276F) might inhibit the function of gap junction channel in a dominant negative manner, but inhibit the hemichannel function in a recessive negative manner.
Precautions
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Disclaimer
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