Full Product Name
GJA8 siRNA (Mouse)
Product Synonym Names
Gap junction alpha-8 protein; Connexin-50; Cx50; Lens fiber protein MP70
Product Gene Name
GJA8 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P28236
Specificity
GJA8 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse GJA8 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of GJA8 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
GJA8 sirna
siRNA to inhibit GJA8 expression using RNA interference
Applications Tested/Suitable for GJA8 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for GJA8. It may not necessarily be applicable to this product.
NCBI Accession #
NP_032149.1
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NCBI GenBank Nucleotide #
NM_008123.3
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UniProt Primary Accession #
P28236
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UniProt Related Accession #
P28236[Other Products]
Molecular Weight
49,598 Da
NCBI Official Full Name
gap junction alpha-8 protein
NCBI Official Synonym Full Names
gap junction protein, alpha 8
NCBI Official Symbol
Gja8 [Similar Products]
NCBI Official Synonym Symbols
Aey5; Cx50; Cnx50; Lop10
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NCBI Protein Information
gap junction alpha-8 protein
UniProt Protein Name
Gap junction alpha-8 protein
UniProt Synonym Protein Names
Connexin-50; Cx50; Lens fiber protein MP70
Protein Family
Gap junction alpha-8 protein
UniProt Gene Name
Gja8 [Similar Products]
UniProt Synonym Gene Names
Cx50 [Similar Products]
UniProt Entry Name
CXA8_MOUSE
UniProt Comments for GJA8
GJA8: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. A connexon is composed of a hexamer of connexins. This particular connexin only forms junctional channels. Eye lens. Belongs to the connexin family. Alpha-type (group II) subfamily.
Protein type: Membrane protein, multi-pass; Membrane protein, integral; Channel, misc.
Cellular Component: connexon complex; membrane; gap junction; integral to membrane; plasma membrane; cell junction
Molecular Function: gap junction channel activity
Biological Process: lens development in camera-type eye; camera-type eye development; cell-cell signaling; cell communication; transmembrane transport; protein homooligomerization
Research Articles on GJA8
1. demonstrate, at the whole gap junction channel level, a crucial role of the surface charge properties in the first transmembrane/first extracellular border domain in determining the efficiency of ion permeation and the Vj gating of Cx50
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
