Full Product Name
BLM siRNA (Human)
Product Synonym Names
RECQ2; RECQL3; Bloom syndrome protein; DNA helicase. RecQ-like type 2; RecQ2; RecQ protein-like 3
Product Gene Name
BLM sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P54132
Specificity
BLM siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human BLM gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of BLM sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
BLM sirna
siRNA to inhibit BLM expression using RNA interference
Applications Tested/Suitable for BLM sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for BLM. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000048.1
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NCBI GenBank Nucleotide #
NM_000057.3
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UniProt Primary Accession #
P54132
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UniProt Secondary Accession #
Q52M96[Other Products]
UniProt Related Accession #
P54132[Other Products]
Molecular Weight
159,000 Da
NCBI Official Full Name
Bloom syndrome protein isoform 1
NCBI Official Synonym Full Names
Bloom syndrome, RecQ helicase-like
NCBI Official Symbol
BLM [Similar Products]
NCBI Official Synonym Symbols
BS; RECQ2; RECQL2; RECQL3
[Similar Products]
NCBI Protein Information
Bloom syndrome protein
UniProt Protein Name
Bloom syndrome protein
UniProt Synonym Protein Names
DNA helicase, RecQ-like type 2; RecQ2; RecQ protein-like 3
Protein Family
Bloom syndrome protein
UniProt Gene Name
BLM [Similar Products]
UniProt Synonym Gene Names
RECQ2; RECQL3; RecQ2 [Similar Products]
UniProt Entry Name
BLM_HUMAN
NCBI Summary for BLM
The Bloom syndrome gene product is related to the RecQ subset of DExH box-containing DNA helicases and has both DNA-stimulated ATPase and ATP-dependent DNA helicase activities. Mutations causing Bloom syndrome delete or alter helicase motifs and may disable the 3'-5' helicase activity. The normal protein may act to suppress inappropriate recombination. [provided by RefSeq, Jul 2008]
UniProt Comments for BLM
BLM: a magnesium-dependent ATP-dependent DNA-helicase that unwinds single- and double-stranded DNA in a 3'-5' direction. A member of the RecQ helicase family that is required for genome stability. Participates in DNA replication, recombination and repair. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex which is a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with ubiquitinated FANCD2.
Protein type: DNA repair, damage; DNA replication; Helicase; EC 3.6.4.12
Chromosomal Location of Human Ortholog: 15q26.1
Cellular Component: chromosome, telomeric region; cytoplasm; lateral element; nuclear chromosome; nuclear matrix; nucleolus; nucleoplasm; nucleus; PML body
Molecular Function: ATP binding; ATP-dependent 3'-5' DNA helicase activity; ATP-dependent DNA helicase activity; ATP-dependent helicase activity; ATPase activity; bubble DNA binding; four-way junction helicase activity; G-quadruplex DNA binding; helicase activity; p53 binding; protein binding; single-stranded DNA binding
Biological Process: cellular protein metabolic process; DNA double-strand break processing; DNA duplex unwinding; DNA recombination; DNA repair; DNA strand renaturation; double-strand break repair; double-strand break repair via homologous recombination; double-strand break repair via synthesis-dependent strand annealing; mitotic cell cycle G2/M transition DNA damage checkpoint; negative regulation of cell division; negative regulation of DNA recombination; positive regulation of transcription, DNA-dependent; post-translational protein modification; protein oligomerization; protein sumoylation; regulation of cyclin-dependent protein kinase activity; replication fork processing; replication fork protection; response to DNA damage stimulus; response to X-ray
Disease: Bloom Syndrome
Research Articles on BLM
1. this is the first genetic association study to show the significant association between BLM gene and Prostate cancer susceptibility in Chinese population.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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