Bloom syndrome, ELISA Kit

BS; RECQ2; RECQL2; RECQL3; RecQ Helicase-Like; DNA helicase, RecQ-like type 2

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Mouse BLM. No significant cross-reactivity or interference between Mouse BLM and analogues was observed.

Store at 2-8 degree C.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of BLM elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS284562 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Bloom syndrome (BLM) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing BLM. The ELISA analytical biochemical technique of the MBS284562 kit is based on BLM antibody-BLM antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BLM antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BLM. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate BLM in samples. An antibody specific for BLM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyBLM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BLM is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BLM bound in the initial step. The color development is stopped and the intensity of the color is measured.

158,366 Da

Bloom syndrome, RecQ like helicase

Bloom syndrome protein homolog

Bloom syndrome protein homolog

mBLM  [Similar Products]

BLM: a magnesium-dependent ATP-dependent DNA-helicase that unwinds single- and double-stranded DNA in a 3'-5' direction. A member of the RecQ helicase family that is required for genome stability. Participates in DNA replication, recombination and repair. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex which is a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with ubiquitinated FANCD2.

Protein type: DNA repair, damage; DNA replication; EC 3.6.4.12; Helicase

Chromosomal Location of Human Ortholog: 7 D2|7 45.65 cM

Cellular Component: chromosome, telomeric region; cytoplasm; cytosol; intracellular; lateral element; male germ cell nucleus; nuclear chromosome; nuclear matrix; nucleolus; nucleus; PML body; pronucleus; replication fork

Molecular Function: ATP binding; ATP-dependent 3'-5' DNA helicase activity; ATP-dependent DNA helicase activity; ATP-dependent helicase activity; ATPase activity; bubble DNA binding; catalytic activity; DNA binding; DNA helicase activity; DNA-dependent ATPase activity; forked DNA-dependent helicase activity; four-way junction DNA binding; four-way junction helicase activity; G-quadruplex DNA binding; helicase activity; hydrolase activity; hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides; metal ion binding; nucleic acid binding; nucleotide binding; p53 binding; protein binding; protein homodimerization activity; single-stranded DNA binding; telomeric D-loop binding; Y-form DNA binding; zinc ion binding

Biological Process: alpha-beta T cell differentiation; cellular metabolic process; cellular response to DNA damage stimulus; cellular response to ionizing radiation; chromosome organization and biogenesis; DNA double-strand break processing; DNA duplex unwinding; DNA recombination; DNA repair; DNA replication; G-quadruplex DNA unwinding; mitotic cell cycle G2/M transition DNA damage checkpoint; negative regulation of cell division; negative regulation of DNA recombination; negative regulation of mitotic recombination; positive regulation of alpha-beta T cell proliferation; positive regulation of double-strand break repair via homologous recombination; positive regulation of transcription, DNA-templated; protein homooligomerization; protein oligomerization; regulation of binding; regulation of cyclin-dependent protein kinase activity; regulation of DNA-dependent DNA replication; replication fork processing; replication-born double-strand break repair via sister chromatid exchange; response to X-ray; telomere maintenance; telomeric D-loop disassembly

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.