Product Name
Bloom Syndrome (BLM), Polyclonal Antibody
Full Product Name
Bloom Syndrome (Phospho-Thr99) Antibody
Product Synonym Names
Bloom syndrome protein; RecQ protein-like 3; DNA helicase; RecQ-like type 2; BLM; RECQ2; RECQL3
Product Gene Name
anti-BLM antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P54132
Specificity
Bloom Syndrome (Phospho-Thr99) Antibody detects endogenous levels of Bloom Syndrome only when phosphorylated at Thr99.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (lot specific)
Target Modification
Phospho
Immunogen
The antiserum was produced against synthesized peptide derived from human Bloom Syndrome around the phosphorylation site of Thr99.
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Other Notes
Small volumes of anti-BLM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-BLM antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-BLM antibody
WB: 1:500~1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:5000
Immunofluorescence (IF) of anti-BLM antibody
Immunofluorescence analysis of HeLa cells, using Bloom Syndrome (Phospho-Thr99) Antibody. The picture on the right is blocked with the phospho peptide.
Immunohistochemistry (IHC) of anti-BLM antibody
Immunohistochemistry analysis of paraffin-embedded human heart, using Bloom Syndrome (Phospho-Thr99) Antibody. The picture on the right is blocked with the phospho peptide.
Western Blot (WB) of anti-BLM antibody
Western blot analysis of lysates from HepG2 cells, using Bloom Syndrome (Phospho-Thr99) Antibody. The lane on the right is blocked with the phospho peptide.
NCBI/Uniprot data below describe general gene information for BLM. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000048.1
[Other Products]
NCBI GenBank Nucleotide #
NM_000057.3
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UniProt Primary Accession #
P54132
[Other Products]
UniProt Secondary Accession #
Q52M96[Other Products]
UniProt Related Accession #
P54132[Other Products]
NCBI Official Full Name
Bloom syndrome protein isoform 1
NCBI Official Synonym Full Names
Bloom syndrome RecQ like helicase
NCBI Official Symbol
BLM [Similar Products]
NCBI Official Synonym Symbols
BS; RECQ2; RECQL2; RECQL3
[Similar Products]
NCBI Protein Information
Bloom syndrome protein
UniProt Protein Name
Bloom syndrome protein
UniProt Synonym Protein Names
DNA helicase, RecQ-like type 2; RecQ2; RecQ protein-like 3
Protein Family
Bloom syndrome protein
UniProt Gene Name
BLM [Similar Products]
UniProt Synonym Gene Names
RECQ2; RECQL3; RecQ2 [Similar Products]
NCBI Summary for BLM
The Bloom syndrome gene product is related to the RecQ subset of DExH box-containing DNA helicases and has both DNA-stimulated ATPase and ATP-dependent DNA helicase activities. Mutations causing Bloom syndrome delete or alter helicase motifs and may disable the 3'-5' helicase activity. The normal protein may act to suppress inappropriate recombination. [provided by RefSeq, Jul 2008]
UniProt Comments for BLM
ATP-dependent DNA helicase that unwinds single- and double-stranded DNA in a 3'-5' direction (PubMed:9388193, PubMed:24816114, PubMed:25901030). Participates in DNA replication and repair (PubMed:12019152, PubMed:21325134, PubMed:23509288). Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA (PubMed:21325134). Negatively regulates sister chromatid exchange (SCE) (PubMed:25901030). Stimulates DNA 4-way junction branch migration and DNA Holliday junction dissolution (PubMed:25901030). Binds single-stranded DNA (ssDNA), forked duplex DNA and DNA Holliday junction (PubMed:20639533, PubMed:24257077, PubMed:25901030).
Research Articles on BLM
1. Mutations within the domain VI of BLM detected in human cancer samples demonstrate the functional importance of this domain, suggesting human tumorigenicity resulting from mtBLM may be at least partly attributed to mitigated FANCD2 activation.
Precautions
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Disclaimer
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