LDLR, Monoclonal Antibody

Anti -LDLR (Low-density Lipoprotein Receptor, LDL Receptor) (PE)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 19; NC_000019.9 (11200037..11244506). Location: 19p13.2

Monoclonal

IgG1

11C32

Mouse

Recognizes human LDLR.

Purified by Protein G affinity chromatography form hybridoma culture supernatant.

Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).

0.025 mg/mL (lot specific)

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Small volumes of anti-LDLR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

LDL R is the prototype low density lipoprotein receptor. It is a transmembrane protein that plays a role in cholesterol homeostasis by mediating the uptake of low density lipoproteins. Some of the many allelic variants of LDL R cause familial hypercholesterolemia.

Antibodies; Abs to Lipoproteins

Flow Cytometry (FC/FACS)

Suitable for use in Flow Cytometry.
Dilution: Flow Cytometry: 10ul labels 1x10e6 cells. Validated using HepG2 cells.

Flow Cytometry Protocol: 1. Cells may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction.
2. After blocking, add 10ul of L2601-02G to up to 1x10e6 cells and incubate for 30 minutes at RT.
3. Remove unbound antibody by washing the cells twice in Flow Cytometry Staining Buffer (F4568-10). Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Human Lyse Buffer.
4. Resuspend the cells in Flow Cytometry Staining Buffer for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with PE-labeled mouse IgG1 antibody.

HepG2 cells (filled histogram) using MBS609177 or isotype control (filled histogram). Cell surface expression of LDL Receptor was determined by flow cytometry using 488nm wavelength excitation and monitoring emitted fluorescence with a detector optimized to collect peak emissions at 565 - 605nm.

95,376 Da[Similar Products]

low density lipoprotein receptor

low-density lipoprotein receptor; LDL receptor; low-density lipoprotein receptor class A domain-containing protein 3

Low-density lipoprotein receptor

LDL receptor  [Similar Products]

LDLR_HUMAN

The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Sep 2010]

LDLR: Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells. Defects in LDLR are the cause of familial hypercholesterolemia (FH); a common autosomal semi- dominant disease that affects about 1 in 500 individuals. The receptor defect impairs the catabolism of LDL, and the resultant elevation in plasma LDL-cholesterol promotes deposition of cholesterol in the skin (xanthelasma), tendons (xanthomas), and coronary arteries (atherosclerosis). Belongs to the LDLR family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: Golgi apparatus; cell surface; membrane; integral to plasma membrane; lysosome; late endosome; early endosome; plasma membrane; coated pit; endosome membrane; receptor complex; external side of plasma membrane

Molecular Function: very-low-density lipoprotein receptor activity; low-density lipoprotein receptor activity; protein binding; low-density lipoprotein binding; clathrin heavy chain binding; calcium ion binding; glycoprotein binding

Biological Process: phototransduction, visible light; lipoprotein catabolic process; cholesterol metabolic process; receptor-mediated endocytosis; cholesterol homeostasis; cholesterol transport; viral reproduction; cholesterol absorption; endocytosis; lipoprotein metabolic process; phospholipid transport; lipid metabolic process; retinoid metabolic process

Disease: Hypercholesterolemia, Familial

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.