Full Product Name
STF-1 (Ab-203) Antibody
Product Synonym Names
Steroidogenic factor 1; NR5A1; AD4BP; FTZF1; SF1
Product Gene Name
anti-STF-1 antibody
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q13285
Species Reactivity
Human, Mouse, Rat
Specificity
STF-1 (Ab-203) antibody detects endogenous levels of total STF-1 protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human STF-1 around the phosphorylation site of serine 203 (Y-A-SP-P-P).
Preparation and Storage
Store at-20 degree C for 1 year.
Other Notes
Small volumes of anti-STF-1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-STF-1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-STF-1 antibody
WB: 1:500~1:1000
ELISA: 1:5000
Western Blot (WB) of anti-STF-1 antibody
Western blot analysis of extracts from rat lung, using STF-1 (Ab-203) antibody.
NCBI/Uniprot data below describe general gene information for STF-1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_004950.2
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NCBI GenBank Nucleotide #
NM_004959.4
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UniProt Primary Accession #
Q13285
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UniProt Secondary Accession #
O15196; Q5T6F5[Other Products]
UniProt Related Accession #
Q13285[Other Products]
Molecular Weight
51,636 Da
NCBI Official Full Name
steroidogenic factor 1
NCBI Official Synonym Full Names
nuclear receptor subfamily 5 group A member 1
NCBI Official Symbol
NR5A1 [Similar Products]
NCBI Official Synonym Symbols
ELP; SF1; FTZ1; POF7; SF-1; AD4BP; FTZF1; SPGF8; SRXX4; SRXY3; hSF-1
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NCBI Protein Information
steroidogenic factor 1
UniProt Protein Name
Steroidogenic factor 1
UniProt Synonym Protein Names
Adrenal 4-binding protein; Fushi tarazu factor homolog 1; Nuclear receptor subfamily 5 group A member 1; Steroid hormone receptor Ad4BP
UniProt Gene Name
NR5A1 [Similar Products]
UniProt Synonym Gene Names
AD4BP; FTZF1; SF1; SF-1; STF-1; hSF-1 [Similar Products]
NCBI Summary for STF-1
The protein encoded by this gene is a transcriptional activator involved in sex determination. The encoded protein binds DNA as a monomer. Defects in this gene are a cause of XY sex reversal with or without adrenal failure as well as adrenocortical insufficiency without ovarian defect. [provided by RefSeq, Jul 2008]
UniProt Comments for STF-1
Transcriptional activator. Essential for sexual differentiation and formation of the primary steroidogenic tissues (PubMed:27378692). Binds to the Ad4 site found in the promoter region of steroidogenic P450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the AMH/Muellerian inhibiting substance gene as well as the AHCH and STAR genes. 5'-YCAAGGYC-3' and 5'-RRAGGTCA-3' are the consensus sequences for the recognition by NR5A1 (PubMed:27378692). The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional activity. Binds phosphatidylcholine (). Binds phospholipids with a phosphatidylinositol (PI) headgroup, in particular PI(3,4)P2 and PI(3,4,5)P3. Activated by the phosphorylation of NR5A1 by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.
Product References and Citations for anti-STF-1 antibody
Santa Barbara P., Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases. Oba K., Biochem. Biophys. Res. Commun. 226:261-267(1996). Hammer G.D., Mol. Cell 3:521-526(1999).
Research Articles on STF-1
1. We provide new evidence of this involvement, describing a novel heterozygous non-sense NR5A1 mutation in a 46,XY-DSD with polysplenia female proband and her father, who had hypospadias and asplenia.
Precautions
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