Adiponectin, ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of ADIPOQ elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2888175 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Adiponectin (ADIPOQ) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ADIPOQ. The ELISA analytical biochemical technique of the MBS2888175 kit is based on ADIPOQ antibody-ADIPOQ antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADIPOQ antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADIPOQ. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

26,414 Da

adiponectin, C1Q and collagen domain containing

adiponectin

Adiponectin

ACDC; ACRP30; APM1; GBP28; ACRP30; apM-1  [Similar Products]

This gene is expressed in adipose tissue exclusively. It encodes a protein with similarity to collagens X and VIII and complement factor C1q. The encoded protein circulates in the plasma and is involved with metabolic and hormonal processes. Mutations in this gene are associated with adiponectin deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Apr 2010]

adiponectin: Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW. Homomultimer. Forms trimers, hexamers and 12- to 18-mers. The trimers (low molecular weight complexes / LMW) are assembled via non-covalent interactions of the collagen-like domains in a triple helix and hydrophobic interactions within the globular C1q domain. Several trimers can associate to form disulfide-linked hexamers (middle molecular weight complexes / MMW) and larger complexes (higher molecular weight / HMW). The HMW-complex assembly may rely aditionally on lysine hydroxylation and glycosylation. LMW, MMW and HMW complexes bind to HBEGF, MMW and HMW complexes bind to PDGFB, and HMW complex binds to FGF2. Interacts with CTRP9A via the C1q domain (heterotrimeric complex). Synthesized exclusively by adipocytes and secreted into plasma.

Protein type: Endoplasmic reticulum; Hormone; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3q27.3

Cellular Component: cell surface; collagen; endoplasmic reticulum; extracellular region; extracellular space

Molecular Function: cytokine activity; hormone activity; identical protein binding; protein binding; protein homodimerization activity; receptor binding; sialic acid binding

Biological Process: adiponectin-activated signaling pathway; brown fat cell differentiation; cellular response to insulin stimulus; circadian rhythm; fatty acid beta-oxidation; fatty acid oxidation; generation of precursor metabolites and energy; glucose homeostasis; glucose metabolic process; membrane depolarization; membrane hyperpolarization; negative regulation of blood pressure; negative regulation of cell migration; negative regulation of fat cell differentiation; negative regulation of gluconeogenesis; negative regulation of granulocyte differentiation; negative regulation of heterotypic cell-cell adhesion; negative regulation of hormone secretion; negative regulation of I-kappaB kinase/NF-kappaB signaling; negative regulation of inflammatory response; negative regulation of low-density lipoprotein receptor biosynthetic process; negative regulation of macrophage differentiation; negative regulation of MAP kinase activity; negative regulation of phagocytosis; negative regulation of protein autophosphorylation; negative regulation of smooth muscle cell migration; negative regulation of smooth muscle cell proliferation; negative regulation of synaptic transmission; negative regulation of transcription, DNA-dependent; negative regulation of tumor necrosis factor production; positive regulation of blood pressure; positive regulation of cellular protein metabolic process; positive regulation of fatty acid metabolic process; positive regulation of glucose import; positive regulation of I-kappaB kinase/NF-kappaB signaling; positive regulation of interleukin-8 production; positive regulation of myeloid cell apoptotic process; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of protein phosphorylation; positive regulation of signal transduction; protein homooligomerization; regulation of fatty acid biosynthetic process; response to activity; response to ethanol; response to glucocorticoid stimulus; response to glucose stimulus; response to hypoxia; response to nutrient; response to sucrose

Disease: Adiponectin, Serum Level Of, Quantitative Trait Locus 1

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