Product Name
PFKM, Recombinant Protein
Full Product Name
PFKM, 1-780aa, Human, His tag, E Coli
Product Synonym Names
6-Phosphofructokinase muscle type; GSD7; PFK-1; PFK1; PFKA; PFKX; 6-Phosphofructokinase; muscle type 6 Phosphofructokinase Muscle Type; EC 2.7.1.11; MGC8699; PFKL; PFKM; PFKP; Phosphofructo 1 Kinase Isozyme A; Phosphofructokinase 1; Phosphofructokinase M; Phosphofructokinase; muscle; Phosphofructokinase; muscle type; Phosphofructokinase; polypeptide X; Phosphohexokinase.
Product Gene Name
PFKM recombinant protein
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
MGSSHHHHHH SSGLVPRGSH MTHEEHHAAK TLGIGKAIAV LTSGGDAQGM NAAVRAVVRV GIFTGARVFF VHEGYQGLVD GGDHIKEATW ESVSMMLQLG GTVIGSARCK DFREREGRLR AAYNLVKRGI TNLCVIGGDG SLTGADTFRS EWSDLLSDLQ KAGKITDEEA TKSSYLNIVG LVGSIDNDFC GTDMTIGTDS ALHRIMEIVD AITTTAQSHQ RTFVLEVMGR HCGYLALVTS LSCGADWVFI PECPPDDDWE EHLCRRLSET RTRGSRLNII IVAEGAIDKN GKPITSEDIK NLVVKRLGYD TRVTVLGHVQ RGGTPSAFDR ILGSRMGVEA VMALLEGTPD TPACVVSLSG NQAVRLPLME CVQVTKDVTK AMDEKKFDEA LKLRGRSFMN NWEVYKLLAH VRPPVSKSGS HTVAVMNVGA PAAGMNAAVR STVRIGLIQG NRVLVVHDGF EGLAKGQIEE AGWSYVGGWT GQGGSKLGTK RTLPKKSFEQ ISANITKFNI QGLVIIGGFE AYTGGLELME GRKQFDELCI PFVVIPATVS NNVPGSDFSV GADTALNTIC TTCDRIKQSA AGTKRRVFII ETMGGYCGYL ATMAGLAAGA DAAYIFEEPF TIRDLQANVE HLVQKMKTTV KRGLVLRNEK CNENYTTDFI FNLYSEEGKG IFDSRKNVLG HMQQGGSPTP FDRNFATKMG AKAMNWMSGK IKESYRNGRI FANTPDSGCV LGMRKRALVF QPVAELKDQT DFEHRIPKEQ WWLKLRPILK ILAKYEIDLD TSDHAHLEHI TRKRSGEAAV
3D Structure
ModBase 3D Structure for P08237
Purity/Purification
> 80% by SDS-PAGE
Form/Format
Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 5mM DTT, 0.2 M NaCl, and 20% glycerol
Concentration
0.5 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Other Notes
Small volumes of PFKM recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
PFKM recombinant protein
PFKM is regulatory glycolytic enzymes that convert fructose 6-phosphate and ATP into fructose 1,6-bisphosphate (through PFK-1), fructose 2,6-bisphosphate (through PFK-2) and ADP. Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease. Recombinant human PFKM protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for PFKM recombinant protein
Cancer
Applications Tested/Suitable for PFKM recombinant protein
SDS-PAGE
SDS-Page of PFKM recombinant protein
NCBI/Uniprot data below describe general gene information for PFKM. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000280
[Other Products]
NCBI GenBank Nucleotide #
NM_000289.5
[Other Products]
UniProt Primary Accession #
P08237
[Other Products]
UniProt Secondary Accession #
Q16814; Q16815; Q6ZTT1; J3KNX3[Other Products]
UniProt Related Accession #
P08237[Other Products]
Molecular Weight
87.3 kDa (800aa), confirmed by MALDI-TOF
NCBI Official Full Name
ATP-dependent 6-phosphofructokinase, muscle type isoform 2
NCBI Official Synonym Full Names
phosphofructokinase, muscle
NCBI Official Symbol
PFKM [Similar Products]
NCBI Official Synonym Symbols
GSD7; PFK1; PFKA; PFKX; PFK-1; PFK-A; ATP-PFK; PPP1R122
[Similar Products]
NCBI Protein Information
ATP-dependent 6-phosphofructokinase, muscle type
UniProt Protein Name
ATP-dependent 6-phosphofructokinase, muscle type
UniProt Synonym Protein Names
6-phosphofructokinase type A; Phosphofructo-1-kinase isozyme A; PFK-A; Phosphohexokinase
Protein Family
ATP-dependent 6-phosphofructokinase
UniProt Gene Name
PFKM [Similar Products]
UniProt Synonym Gene Names
PFKX; ATP-PFK; PFK-M [Similar Products]
NCBI Summary for PFKM
Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Tetramer composition varies depending on tissue type. This gene encodes the muscle-type isozyme. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease. Alternatively spliced transcript variants have been described.[provided by RefSeq, Nov 2009]
UniProt Comments for PFKM
Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
Product References and Citations for PFKM recombinant protein
Martinez-Costa OH., et al. (2007) FEBS Lett. 581 (16):3033-8. ; Kim JW., et al. (2004) Mol Cell Biol. 24 (13):5923-36.;
Research Articles on PFKM
1. Data indicate that 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase3 (PFKFB3) and phosphofructokinase (PFK1) expression allows discrimination between induced pluripotent stem cells (iPS) and cancer stem cells (CSCs).
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
