regulatory factor X-associated ankyrin-containing protein, ELISA Kit

Human DNA-binding protein RFXANK (RFXANK) ELISA kit; ANKRA1; BLS; F14150_1; MGC138628; RFX-B; DNA-binding protein RFXANK; RFX-Bdelta4; ankyrin repeat-containing regulatory factor X-associated protein; regulatory factor X subunit B; regulatory factor X-associated ankyrin-containing protein

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C

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Small volumes of RFXANK elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9318104 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the regulatory factor X-associated ankyrin-containing protein (RFXANK) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing RFXANK. The ELISA analytical biochemical technique of the MBS9318104 kit is based on RFXANK antibody-RFXANK antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RFXANK antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RFXANK. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

28,102 Da

regulatory factor X-associated ankyrin-containing protein

DNA-binding protein RFXANK; RFX-Bdelta4; regulatory factor X subunit B; ankyrin repeat family A protein 1; ankyrin repeat-containing regulatory factor X-associated protein

DNA-binding protein RFXANK

ANKRA1; RFXB; RFX-B  [Similar Products]

RFXK_HUMAN

Major histocompatibility (MHC) class II molecules are transmembrane proteins that have a central role in development and control of the immune system. The protein encoded by this gene, along with regulatory factor X-associated protein and regulatory factor-5, forms a complex that binds to the X box motif of certain MHC class II gene promoters and activates their transcription. Once bound to the promoter, this complex associates with the non-DNA-binding factor MHC class II transactivator, which controls the cell type specificity and inducibility of MHC class II gene expression. This protein contains ankyrin repeats involved in protein-protein interactions. Mutations in this gene have been linked to bare lymphocyte syndrome type II, complementation group B. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2013]

RFXANK: Activates transcription from class II MHC promoters. Activation requires the activity of the MHC class II transactivator (MHC2TA). May regulate other genes in the cell. RFX binds the X1 box of MHC-II promoters. Isoform RFX-B-delta5 is not involved in the positive regulation of MHC class II genes. Defects in RFXANK are a cause of bare lymphocyte syndrome type 2 (BLS2); also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency. BLS2 is a severe combined immunodeficiency disease with early onset. It is characterized by a profound defect in constitutive and interferon-gamma induced MHC II expression, absence of cellular and humoral T-cell response to antigen challenge, hypogammaglobulinemia and impaired antibody production. The consequence include extreme susceptibility to viral, bacterial and fungal infections. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor; DNA-binding

Chromosomal Location of Human Ortholog: 19p12

Cellular Component: nucleoplasm; intercellular bridge; cytoplasm

Molecular Function: DNA binding; histone deacetylase binding; transcription cofactor activity; transcription factor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; Ras protein signal transduction; positive regulation of transcription from RNA polymerase II promoter

Disease: Bare Lymphocyte Syndrome, Type Ii

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