ESPL1, Polyclonal Antibody

ESP1; SEPA

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

Antigen affinity purification

2.1mg/mL (lot specific)

Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Manufactured in an ISO 9001:2015 Certified Laboratory.

Small volumes of anti-ESPL1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 or STAG2 in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 by ESPL1, or separase, which initiates the final separation of sister chromatids.

Cell biology

ELISA (EIA), Immunohistochemistry (IHC)

IHC: 1:50-1:200

Immunohistochemistry of paraffin-embedded Human gasrtic cancer tissue using ESPL1 Polyclonal Antibody at dilution 1:40

Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using ESPL1 Polyclonal Antibody at dilution 1:40

233,035 Da

extra spindle pole bodies 1 (S. cerevisiae)

separin; Cut1/ESP1 related protein; caspase-like protein ESPL1; extra spindle poles-like 1 protein; separase

Separin

Esp1; Kiaa0165  [Similar Products]

ESPL1_MOUSE

separase: a caspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by different mechanisms. Regulated by at least two independent mechanisms. First, it is inactivated via its interaction with securin/PTTG1, which probably covers its active site. Second, phosphorylation inactivates it. It is completely phosphorylated during mitosis and dephosphorylated during anaphase. Two alternatively spliced isoforms have been described.

Protein type: EC 3.4.22.49; Protease

Cellular Component: centrosome; cytoplasm; nucleus

Molecular Function: peptidase activity; hydrolase activity; cysteine-type peptidase activity

Biological Process: mitosis; homologous chromosome segregation; mitotic sister chromatid segregation; proteolysis; meiotic spindle organization and biogenesis; meiosis I; chromosome segregation

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