Probable cation-transporting ATPase 13A2, ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Human ATP13A2. No significant cross-reactivity or interference between Human ATP13A2 and analogues was observed.

Store at 2-8 degree C.

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Small volumes of ATP13A2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS281444 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Probable cation-transporting ATPase 13A2 (ATP13A2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ATP13A2. The ELISA analytical biochemical technique of the MBS281444 kit is based on ATP13A2 antibody-ATP13A2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ATP13A2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ATP13A2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ATP13A2 in samples. An antibody specific for ATP13A2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyATP13A2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ATP13A2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ATP13A2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

128,323 Da

ATPase 13A2

cation-transporting ATPase 13A2; probable cation-transporting ATPase 13A2

Cation-transporting ATPase 13A2

This gene encodes a member of the P5 subfamily of ATPases which transports inorganic cations as well as other substrates. Mutations in this gene are associated with Kufor-Rakeb syndrome (KRS), also referred to as Parkinson disease 9. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Nov 2008]

ATP13A2: May play a role in intracellular cation homeostasis and the maintenance of neuronal integrity. Expressed in brain; protein levels are markedly increased in brain from subjects with Parkinson disease and subjects with dementia with Lewy bodies. Detected in pyramidal neurons located throughout the cingulate cortex. In the substantia nigra, it is found in neuromelanin- positive dopaminergic neurons. Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type V subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.6.3.-; Hydrolase; Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, ion channel

Chromosomal Location of Human Ortholog: 1p36.13

Cellular Component: autophagic vacuole; cell soma; integral component of membrane; integral component of plasma membrane; late endosome; lysosomal lumen; lysosomal membrane; lysosome; multivesicular body; neuron projection; transport vesicle; vesicle; vesicle membrane

Molecular Function: ATP binding; ATPase activity; calcium-transporting ATPase activity; cation-transporting ATPase activity; manganese ion binding; protein binding; zinc ion binding

Biological Process: cellular calcium ion homeostasis; cellular cation homeostasis; cellular iron ion homeostasis; cellular zinc ion homeostasis; positive regulation of protein secretion; protein autophosphorylation; regulation of autophagic vacuole size; regulation of endopeptidase activity; regulation of intracellular protein transport; regulation of macroautophagy; zinc ion homeostasis

Disease: Kufor-rakeb Syndrome; Spastic Paraplegia 78, Autosomal Recessive

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