TRPV4, Polyclonal Antibody

Rabbit Polyclonal (IgG) to Human TRPV4; Human TRPV4; TRPV4; CMT2C; HMSN2C; OTRPC4; SSQTL1; VR-OAC; VRL-2; Osm-9-like TRP channel 4; SPSMA; TRP12; VRL2; VROAC; SMAL

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

TRPV4 antibody is human specific. At least six isoforms of TRPV4 are known to exist. This antibody is predicted to not cross-react with other TRP protein family members.

Immunoaffinity purified

PBS, 0.02% sodium azide.

1 mg/ml (lot specific)

Long term: -20 degree C
Short term: 4 degree C
Avoid repeat freeze-thaw cycles

Small volumes of anti-TRPV4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

TRPV4 Antibody, CMT2C Antibody, HMSN2C Antibody, OTRPC4 Antibody, SSQTL1 Antibody, VR-OAC Antibody, VRL-2 Antibody, Osm-9-like TRP channel 4 Antibody, SPSMA Antibody, TRP12 Antibody, VRL2 Antibody, VROAC Antibody, SMAL Antibody Description: Non-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca2+ potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism.

Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)

IF (20 ug/ml)
IHC-P (10 ug/ml)
WB (1 - 2 ug/ml)

Anti-TRPV4 antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Anti-TRPV4 antibody IHC staining of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Immunofluorescence of TRPV4 in human lung tissue with TRPV4 antibody at 20 µg/mL.

Western blot analysis of TRPV4 in human testis tissue lysate with TRPV4 antibody at 1 ug/ml.

85,884 Da

transient receptor potential cation channel subfamily V member 4

transient receptor potential cation channel subfamily V member 4

Transient receptor potential cation channel subfamily V member 4

VRL2; VROAC; TrpV4; OTRPC4; TRP12; VRL-2; VR-OAC  [Similar Products]

TRPV4_HUMAN

This gene encodes a member of the OSM9-like transient receptor potential channel (OTRPC) subfamily in the transient receptor potential (TRP) superfamily of ion channels. The encoded protein is a Ca2+-permeable, nonselective cation channel that is thought to be involved in the regulation of systemic osmotic pressure. Mutations in this gene are the cause of spondylometaphyseal and metatropic dysplasia and hereditary motor and sensory neuropathy type IIC. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2010]

TRPV4: a non-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers. Acts as a regulator of intracellular Ca(2+) in synoviocytes. Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4-alpha-phorbol 12,13-didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8. Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV4 sub-subfamily. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Channel, cation; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q24.1

Cellular Component: adherens junction; apical plasma membrane; cell surface; cilium; cortical actin cytoskeleton; cytoplasmic microtubule; cytoplasmic vesicle; cytosol; filopodium; focal adhesion; growth cone; integral to membrane; lamellipodium; plasma membrane

Molecular Function: actin binding; actin filament binding; alpha-tubulin binding; ATP binding; beta-tubulin binding; calcium channel activity; calmodulin binding; cation channel activity; microtubule binding; osmosensor activity; protein binding; protein kinase binding; protein kinase C binding; SH2 domain binding; stretch-activated, cation-selective, calcium channel activity

Biological Process: actin cytoskeleton reorganization; actin filament organization; calcium ion transport; cell volume homeostasis; cellular calcium ion homeostasis; cortical microtubule organization and biogenesis; elevation of cytosolic calcium ion concentration; hyperosmotic salinity response; intercellular junction assembly; microtubule polymerization; multicellular organismal water homeostasis; osmosensory signaling pathway; positive regulation of microtubule depolymerization; positive regulation of vascular permeability; regulation of response to osmotic stress; response to mechanical stimulus; transmembrane transport; vasopressin secretion

Disease: Brachyolmia Type 3; Digital Arthropathy-brachydactyly, Familial; Hereditary Motor And Sensory Neuropathy, Type Iic; Metatropic Dysplasia; Parastremmatic Dwarfism; Scapuloperoneal Spinal Muscular Atrophy; Sodium Serum Level Quantitative Trait Locus 1; Spinal Muscular Atrophy, Distal, Congenital Nonprogressive; Spondyloepiphyseal Dysplasia, Maroteaux Type; Spondylometaphyseal Dysplasia, Kozlowski Type

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