CD4, Monoclonal Antibody

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG2b

GK1.5

Rat

Low Endotoxin
Purified IgG - liquid

IgG concentration 1.0 mg/ml (lot specific)

Store at -20 degree C only. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Small volumes of anti-CD4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Immunohistology Frozen, ELISA (EIA), Flow cytometry (FC/FACS), Functional Assays (FN), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB)

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry: Minimum Dilution: 1/25; Maximum Dilution: 1/200

Staining of mouse spleen with Rat anti Mouse CD4 (MBS219566)

Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD4 antibody, clone GK1.5 (MBS219566) followed by horseradish peroxidase conjugated Goat anti Rat IgG (MBS235195). Medium power

Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD4 antibody, clone GK1.5 (MBS219566) followed by horseradish peroxidase conjugated Goat anti Rat IgG (MBS235195). High power

Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD4 antibody, clone GK1.5 (MBS219566) followed by horseradish peroxidase conjugated Goat anti Rat IgG (MBS235195). Low power

Published customer image: Intrafollicular location of MZB cells in B6.TC spleens. A. Representative spleen sections from 3 mo old B6.TC (left) and B6 (right) mice stained with Moma-1-FITC, CD1d-PE and B220-PB. The B220+ CD1d+ MZB cells show as bright pink while the other B cells show as blue. The ring of Moma-1+ metallophillic macrophages delineates the MZ inner edge. B. Percentage of CD1d+ B220+ B cells relative to total B220+ B cells outside (MZ) and inside (FO) the Moma-1+ ring in 3 mo and 10 mo old B6 and B6.TC mice. The data show means and standard errors of the mean (SEM) calculated of 4 MZ and FO areas for each mouse. ***: p < 0.001 for t tests. C. Representative spleen sections from 10 mo old B6.TC (left) and B6 (right) mice stained with CD4-FITC, CD1d-PE and B220-PB. Boxed areas show multiple contacts between green B6.TC CD4 T cells and pink MZB cells, but not in the B6 spleen. Original magnification: 200X.From: Zhou et al. BMC Immunology 2011 12:7.

Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD4 Alexa Fluor ?647 (MBS219566A647)

Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD4:FITC (MBS219462)

Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD4:Low Endotoxin

Staining of mouse spleen with Rat anti Mouse CD4:Alexa Fluor ?488 (MBS219566A488)

Staining of mouse spleen with Rat anti Mouse CD4:RPE (MBS219761)

24,473 Da

CD4 antigen

T-cell surface glycoprotein CD4; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4)

T-cell surface glycoprotein CD4

CD4_MOUSE

CD4: Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. Associates with LCK. Binds to HIV-1 gp120 and to P4HB/PDI and upon HIV-1 binding to the cell membrane, is part of P4HB/PDI- CD4-CXCR4-gp120 complex. Interacts with HIV-1 Envelope polyprotein gp160 and protein Vpu. Interacts with Human Herpes virus 7 capsid proteins. Interacts with PTK2/FAK1; this interaction requires the presence of HIV-1 gp120.

Protein type: Cell surface; Membrane protein, integral

Cellular Component: endoplasmic reticulum membrane; cell surface; membrane; endoplasmic reticulum lumen; integral to membrane; plasma membrane; external side of plasma membrane; lipid raft

Molecular Function: protein binding; enzyme binding; protein homodimerization activity; zinc ion binding; coreceptor activity; glycoprotein binding; protein kinase binding

Biological Process: maintenance of cellular protein localization; T cell activation; immune system process; T cell selection; cytokine production; positive regulation of calcium-mediated signaling; defense response to Gram-negative bacterium; induction by virus of cell-cell fusion in host; positive regulation of peptidyl-tyrosine phosphorylation; cell surface receptor linked signal transduction; positive regulation of protein kinase activity; protein palmitoleylation; immune response; positive regulation of T cell activation; cell adhesion; T cell differentiation; regulation of T cell activation

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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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