Full Product Name
BMP2 antibody
Product Synonym Names
Bone morphogenetic protein 2; BMP-2A; bmp2; Bone morphogenetic protein-2
Product Gene Name
anti-BMP2 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P12643
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (lot specific)
Immunogen
Recombinant human BMP2 (283-396aa) purified from E. coli
Clone Note
Anti-human BMP2 mAb, clone AT15B3, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human BMP2 protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Other Notes
Small volumes of anti-BMP2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-BMP2 antibody
BMP-2 (Bone morphogenetic protein 2) is multi-functional growth factors that belong to the transforming growth factor beta (TGF beta) superfamily. It plays an important role in embryonic dorsal-ventral patterning, organogenesis, limb bud formation, and bone formation and regeneration. BMP-2 also promotes the maintenance and repair of colonic epithelium, suppresses neuronal dopamine synthesis and release, induces apoptosis in medulloblastoma cells, and is required for cardiac contractility.
Product Categories/Family for anti-BMP2 antibody
Hormones & Cytokines
Applications Tested/Suitable for anti-BMP2 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-BMP2 antibody
Recommended starting dilution is 1:1000.
Western Blot (WB) of anti-BMP2 antibody
The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BMP2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HepG2 cell lysate
Immunofluorescence (IF) of anti-BMP2 antibody
ICC/IF analysis of BMP2 in Hep3B cells line, stained with monoclonal anti-human BMP2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
NCBI/Uniprot data below describe general gene information for BMP2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001191
[Other Products]
NCBI GenBank Nucleotide #
NM_001200.3
[Other Products]
UniProt Primary Accession #
P12643
[Other Products]
UniProt Related Accession #
P12643[Other Products]
Molecular Weight
44,702 Da
NCBI Official Full Name
bone morphogenetic protein 2 preproprotein
NCBI Official Synonym Full Names
bone morphogenetic protein 2
NCBI Official Symbol
BMP2 [Similar Products]
NCBI Official Synonym Symbols
BDA2; BMP2A
[Similar Products]
NCBI Protein Information
bone morphogenetic protein 2
UniProt Protein Name
Bone morphogenetic protein 2
UniProt Synonym Protein Names
Bone morphogenetic protein 2A; BMP-2A
Protein Family
Bone morphogenetic protein
UniProt Gene Name
BMP2 [Similar Products]
UniProt Synonym Gene Names
BMP2A; BMP-2; BMP-2A [Similar Products]
NCBI Summary for BMP2
This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer, which plays a role in bone and cartilage development. Duplication of a regulatory region downstream of this gene causes a form of brachydactyly characterized by a malformed index finger and second toe in human patients. [provided by RefSeq, Jul 2016]
UniProt Comments for BMP2
Induces cartilage and bone formation (PubMed:3201241). Stimulates the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A- ATF4 pathway. BMP2 activation of EIF2AK3 stimulates phosphorylation of EIF2A which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation. In addition stimulates TMEM119, which upregulates the expression of ATF4 (PubMed:24362451).
Product References and Citations for anti-BMP2 antibody
Chen D., et al. (2004). Growth factors. 22 (4):233-41 ; Schliephake H., et al. (2005). Clin Oral Implants Res. 17 (6):666-72
Research Articles on BMP2
1. BMP2-transduced BMSCs can maintain the chondrocyte-like phenotype in PRP gel in vitro, and the combined use of these two agents can significantly promote repair of the degenerated discs in vivo
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
