Full Product Name
HEXB siRNA (Mouse)
Product Synonym Names
Beta-hexosaminidase subunit beta; Beta-N-acetylhexosaminidase subunit beta; Hexosaminidase subunit B; N-acetyl-beta-glucosaminidase subunit beta
Product Gene Name
HEXB sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P20060
Specificity
HEXB siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse HEXB gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of HEXB sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
HEXB sirna
siRNA to inhibit HEXB expression using RNA interference
Applications Tested/Suitable for HEXB sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for HEXB. It may not necessarily be applicable to this product.
NCBI Accession #
NP_034552.1
[Other Products]
NCBI GenBank Nucleotide #
NM_010422.2
[Other Products]
UniProt Primary Accession #
P20060
[Other Products]
UniProt Related Accession #
P20060[Other Products]
Molecular Weight
61,116 Da
NCBI Official Full Name
beta-hexosaminidase subunit beta
NCBI Official Synonym Full Names
hexosaminidase B
NCBI Official Symbol
Hexb [Similar Products]
NCBI Protein Information
beta-hexosaminidase subunit beta
UniProt Protein Name
Beta-hexosaminidase subunit beta
UniProt Synonym Protein Names
Beta-N-acetylhexosaminidase subunit beta; Hexosaminidase subunit B; N-acetyl-beta-glucosaminidase subunit beta
Protein Family
Beta-hexosaminidase
UniProt Gene Name
Hexb [Similar Products]
UniProt Synonym Gene Names
Hexosaminidase subunit B [Similar Products]
UniProt Entry Name
HEXB_MOUSE
UniProt Comments for HEXB
HEXB: Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues. Defects in HEXB are the cause of GM2-gangliosidosis type 2 (GM2G2); also known as Sandhoff disease. GM2- gangliosidosis is an autosomal recessive lysosomal storage disease marked by the accumulation of GM2 gangliosides in the neuronal cells. GM2G2 is clinically indistinguishable from GM2- gangliosidosis type 1, presenting startle reactions, early blindness, progressive motor and mental deterioration, macrocephaly and cherry-red spots on the macula. Belongs to the glycosyl hydrolase 20 family.
Protein type: Hydrolase; Glycan Metabolism - glycosphingolipid biosynthesis - globo series; Glycan Metabolism - glycosaminoglycan degradation; EC 3.2.1.52; Glycan Metabolism - glycosphingolipid biosynthesis - ganglio series; Glycan Metabolism - other glycan degradation; Carbohydrate Metabolism - amino sugar and nucleotide sugar
Cellular Component: membrane; lysosome; cell; acrosome
Molecular Function: protein homodimerization activity; beta-N-acetylglucosaminidase activity; hexosaminidase activity; hydrolase activity; protein heterodimerization activity; beta-N-acetylhexosaminidase activity; hydrolase activity, acting on glycosyl bonds; carbohydrate binding; hydrolase activity, hydrolyzing O-glycosyl compounds
Biological Process: male courtship behavior; oogenesis; myelination; glycosaminoglycan metabolic process; ganglioside catabolic process; metabolic process; locomotory behavior; regulation of cell shape; sequestering of lipid; oligosaccharide catabolic process; sensory perception of sound; single fertilization; penetration of zona pellucida; neuromuscular process controlling balance; skeletal development; neuromuscular process; N-acetylglucosamine metabolic process; phospholipid biosynthetic process; cellular calcium ion homeostasis; cellular protein metabolic process; sexual reproduction; lysosome organization and biogenesis; carbohydrate metabolic process; positive regulation of transcription from RNA polymerase II promoter; glycosphingolipid metabolic process; regulation of cellular metabolic process; astrocyte cell migration
Research Articles on HEXB
1. mast cell granule beta-hexosaminidase is crucial for defense against bacterial invasion, but is not involved in the allergic response. the bactericidal mechanism of beta-hexosaminidase involves degradation of bacterial cell wall peptidoglycan.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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