Voltage dependent L type calcium channel subunit Alpha 1S (CACNA1S), ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of CACNA1S. No significant cross-reactivity or interference between CACNA1S and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CACNA1S and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of CACNA1S elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7242064 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Voltage dependent L type calcium channel subunit Alpha 1S (CACNA1S) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CACNA1S. The ELISA analytical biochemical technique of the MBS7242064 kit is based on CACNA1S antibody-CACNA1S antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CACNA1S antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CACNA1S. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Intended Uses: This CACNA1S ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CACNA1S. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: CACNA1S ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-CACNA1S antibody and an CACNA1S-HRP conjugate. The assay sample and buffer are incubated together with CACNA1S-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CACNA1S concentration since CACNA1S from samples and CACNA1S-HRP conjugate compete for the anti-CACNA1S antibody . Since the number of sites is limited, as more sites are occupied by CACNA1S from the sample, fewer sites are left to bind CACNA1S-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CACNA1S concentration in each sample is interpolated from this standard curve.

212,350 Da

calcium channel, voltage-dependent, L type, alpha 1S subunit

voltage-dependent L-type calcium channel subunit alpha-1S; dihydropyridine receptor; voltage-gated calcium channel subunit alpha Cav1.1; dihydropyridine-sensitive L-type calcium channel alpha-1 subunit; calcium channel, L type, alpha 1 polypeptide, isoform 3 (skeletal muscle, hypokalemic periodic paralysis)

Voltage-dependent L-type calcium channel subunit alpha-1S

CACH1; CACN1; CACNL1A3  [Similar Products]

CAC1S_HUMAN

This gene encodes one of the five subunits of the slowly inactivating L-type voltage-dependent calcium channel in skeletal muscle cells. Mutations in this gene have been associated with hypokalemic periodic paralysis, thyrotoxic periodic paralysis and malignant hyperthermia susceptibility. [provided by RefSeq, Jul 2008]

CACNA1S: Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1S gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin- GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA). Calcium channels containing the alpha-1S subunit play an important role in excitation-contraction coupling in skeletal muscle. Defects in CACNA1S are the cause of periodic paralysis hypokalemic type 1 (HOKPP1); also designated HYPOPP. HOKPP1 is an autosomal dominant disorder manifested by episodic flaccid generalized muscle weakness associated with falls of serum potassium levels. Defects in CACNA1S are the cause of malignant hyperthermia susceptibility type 5 (MHS5); an autosomal dominant disorder that is potentially lethal in susceptible individuals on exposure to commonly used inhalational anesthetics and depolarizing muscle relaxants. Defects in CACNA1S are the cause of susceptibility to thyrotoxic periodic paralysis type 1 (TTPP1). A sporadic muscular disorder characterized by episodic weakness and hypokalemia during a thyrotoxic state. It is clinically similar to hereditary hypokalemic periodic paralysis, except for the fact that hyperthyroidism is an absolute requirement for disease manifestation. The disease presents with recurrent episodes of acute muscular weakness of the four extremities that vary in severity from paresis to complete paralysis. Attacks are triggered by ingestion of a high carbohydrate load or strenuous physical activity followed by a period of rest. Thyrotoxic periodic paralysis can occur in association with any cause of hyperthyroidism, but is most commonly associated with Graves disease. Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1S subfamily.

Protein type: Membrane protein, integral; Channel, calcium; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: I band; sarcoplasmic reticulum; T-tubule; cytoplasm; plasma membrane; voltage-gated calcium channel complex

Molecular Function: voltage-gated calcium channel activity; metal ion binding; high voltage-gated calcium channel activity

Biological Process: endoplasmic reticulum organization and biogenesis; axon guidance; striated muscle contraction; muscle contraction; calcium ion transport; skeletal muscle adaptation; skeletal muscle fiber development; extraocular skeletal muscle development; skeletal development; myoblast fusion; neuromuscular junction development

Disease: Malignant Hyperthermia, Susceptibility To, 5; Thyrotoxic Periodic Paralysis, Susceptibility To, 1; Hypokalemic Periodic Paralysis, Type 1

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.