14-3-3 eta, Recombinant Protein

Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein eta polypeptide; YWHA1; YWHAH; Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein; eta polypeptide; 14-3-3 eta; Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein; eta polypeptide 14 3 3 protein eta; HGNC:12853; Protein AS1; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein eta polypeptide; Tyrosine 3/tryptophan 5 monooxygenase activation protein eta polypeptide; YWHA 1.

For Research Use Only. Not for use in diagnostic procedures.

E Coli

> 95% by SDS-PAGE

Liquid in 20mM Tris pH 8.0, 10% Glycerol.

1 mg/ml (determined by Absorbance at 280nm) (lot specific)

Can be stored at 4°C short term (1-2 weeks).
For long term storage, aliquot and store at -20°C or -70°C.
Avoid repeated freezing and thawing cycles.

Small volumes of 14-3-3 eta recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon,sigma, zeta, tau and eta that have been identified in mammals. The 14-3-3eta (eta), a subtype of the 14-3-3 family of proteins, was found in B cells, brain, cerebrospinal fluid etc. 14-3-3eta interacts with and relocalizes the A20 zinc finger protein from the insoluble to the soluble fraction, suggesting a chaperone function. Recombinant human 14-3-3eta, fused to His-tag at N-terminus, was expressed in E Coli and purified by conventional chromatography techniques.

Signal Transduction

SDS-PAGE

30.3 kDa (266 aa), confirmed by MALDI-TOF.

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein eta

14-3-3 protein eta

14-3-3 protein eta

YWHA1  [Similar Products]

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and bovine orthologs. This gene contains a 7 bp repeat sequence in its 5' UTR, and changes in the number of this repeat have been associated with early-onset schizophrenia and psychotic bipolar disorder. [provided by RefSeq, Jun 2009]

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.

Sato S., et al. (2006) EMBO J 25 (1):211-21; Chen J., et al. (2007), J Neurosci Res. 85 (8):1724-33;

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