14.3.3 (eta), Antibody

For Research Use Only. Not for use in diagnostic procedures.

Affinity Purified - Liquid

Small volumes of anti-14-3-3 eta antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

The 14.3.3 family of proteins were originally discovered as prominent protein spots on 2 dimensional gels. They are a family of 28-33kDa proteins which are often major proteins of the cytoplasm (1). They function as binding partners for phosphoserine and phosphthreonine sites in other proteins, though they have other binding partners which are not dependent on phosphorylation. These binding interactions are important in the regulation of molecules such as the signaling kinases in the MAP kinase pathway c-Raf and b-Raf, the proapoptotic molecules Bad and Bax and the cell cycle regulator Cdc25.

Antibodies; Neuronal-Glial Markers; Neuron/Synapse Markers; 14-3-3 eta

Immunofluorescence (IF), Western Blot (WB)

Image: rat mixed neuron/glial cultures stained with 14-3-3 eta antibody (red). Neuronal perikarya are very rich in 14-3-3 eta which has a diffuse cytoplasmic staining pattern. Blue is a DNA stain. Protocol on data-sheet.

Image: Blots of crude HeLa cell homogenate blotted with 14-3-3 eta (left lane) and various molecular weight standards (right lane- numbers indicate apparent SDS-PAGE molecular weight in kDa). The mouse monoclonal antibody binds strongly and cleanly to a band at about 28kDa. Protocol on Datasheet.

28,219 Da

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein eta

14-3-3 protein eta

14-3-3 protein eta

YWHA1  [Similar Products]

1433F_HUMAN

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and bovine orthologs. This gene contains a 7 bp repeat sequence in its 5' UTR, and changes in the number of this repeat have been associated with early-onset schizophrenia and psychotic bipolar disorder. [provided by RefSeq, Jun 2009]

14-3-3 eta: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes.

Protein type: Nuclear receptor co-regulator; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 22q12.3

Cellular Component: cytoplasmic vesicle membrane; cytoplasm; plasma membrane; cytosol

Molecular Function: insulin-like growth factor receptor binding; protein domain specific binding; protein binding; enzyme binding; sodium channel regulator activity; protein heterodimerization activity; actin binding; glucocorticoid receptor binding

Biological Process: intracellular protein transport; substantia nigra development; apoptosis; regulation of neuron differentiation; positive regulation of transcription, DNA-dependent; glucocorticoid receptor signaling pathway; regulation of synaptic plasticity; glucocorticoid catabolic process; negative regulation of dendrite morphogenesis; regulation of sodium ion transport

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