Product Name
NTRK1, siRNA
Full Product Name
NTRK1 siRNA (Rat)
Product Synonym Names
TRK; TRKA; High affinity nerve growth factor receptor; Neurotrophic tyrosine kinase receptor type 1; Slow nerve growth factor receptor; p140-TrkA; Trk-A
Product Gene Name
NTRK1 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P35739
Specificity
NTRK1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat NTRK1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of NTRK1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
NTRK1 sirna
siRNA to inhibit NTRK1 expression using RNA interference
Applications Tested/Suitable for NTRK1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for NTRK1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_067600.1
[Other Products]
NCBI GenBank Nucleotide #
NM_021589.1
[Other Products]
UniProt Primary Accession #
P35739
[Other Products]
UniProt Related Accession #
P35739[Other Products]
Molecular Weight
87,252 Da
NCBI Official Full Name
high affinity nerve growth factor receptor
NCBI Official Synonym Full Names
neurotrophic tyrosine kinase, receptor, type 1
NCBI Official Symbol
Ntrk1 [Similar Products]
NCBI Official Synonym Symbols
Trk
[Similar Products]
NCBI Protein Information
high affinity nerve growth factor receptor
UniProt Protein Name
High affinity nerve growth factor receptor
UniProt Synonym Protein Names
Neurotrophic tyrosine kinase receptor type 1; Slow nerve growth factor receptor; p140-TrkA; Trk-A
Protein Family
High affinity nerve growth factor receptor
UniProt Gene Name
Ntrk1 [Similar Products]
UniProt Synonym Gene Names
Trk; Trka; Trk-A [Similar Products]
UniProt Entry Name
NTRK1_RAT
NCBI Summary for NTRK1
a protooncogene; receptor that binds to nerve growth factor (NGF) [RGD, Feb 2006]
UniProt Comments for NTRK1
TrkA: a receptor tyrosine kinase of the Trk family. High affinity receptor for nerve growth factor, neurotrophin-3 and neurotrophin-4/5 but not brain- derived neurotrophic factor. Known substrates include Shc, PI-3K, and PLC-gamma-1. Has a crucial role in the development and function of the nociceptive reception system. Two splice variant isoforms have been described.
Protein type: Membrane protein, integral; Protein kinase, tyrosine (receptor); Kinase, protein; Protein kinase, TK; EC 2.7.10.1; Oncoprotein; TK group; Trk family
Cellular Component: protein complex; cell surface; integral to plasma membrane; late endosome membrane; early endosome; dendrite; Golgi membrane; cell soma; axon; early endosome membrane; cytoplasm; late endosome; plasma membrane; cytoplasmic vesicle; receptor complex
Molecular Function: neurotrophin p75 receptor binding; protein binding; nerve growth factor receptor activity; protein homodimerization activity; ephrin receptor binding; protein-tyrosine kinase activity; nerve growth factor binding; transmembrane receptor protein tyrosine kinase activity; kinase binding; ATP binding
Biological Process: circadian rhythm; response to nicotine; axon guidance; mechanoreceptor differentiation; peptidyl-tyrosine phosphorylation; nerve growth factor receptor signaling pathway; protein amino acid autophosphorylation; sensory perception of pain; olfactory nerve development; positive regulation of synaptic transmission, glutamatergic; protein amino acid phosphorylation; activation of NF-kappaB transcription factor; negative regulation of cell proliferation; response to radiation; sympathetic nervous system development; learning and/or memory; negative regulation of neuron apoptosis; response to axon injury; detection of temperature stimulus involved in sensory perception of pain; response to electrical stimulus; response to hydrostatic pressure; aging; response to nutrient levels; response to drug; nervous system development; Sertoli cell development; detection of mechanical stimulus involved in sensory perception of pain; positive regulation of angiogenesis; positive regulation of programmed cell death; response to ethanol; B cell differentiation; positive regulation of Ras protein signal transduction; response to activity
Research Articles on NTRK1
1. USP8 knockdown increases TrkA half-life, suggesting that the deubiquitinating activity of USP8 promotes TrkA degradation.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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