2-acylglycerol O-acyltransferase 2 (MOGAT2), ELISA Kit

DGAT2L5; FLJ22644; MGAT2; MGC119183; MGC119184; MGC119185; acyl CoA:monoacylglycerol acyltransferase 2

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Human MOGAT2. No significant cross-reactivity or interference between Human MOGAT2 and analogues was observed.

Store at 2-8 degree C.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of MOGAT2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS287541 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 2-acylglycerol O-acyltransferase 2 (MOGAT2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MOGAT2. The ELISA analytical biochemical technique of the MBS287541 kit is based on MOGAT2 antibody-MOGAT2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MOGAT2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MOGAT2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Description: This assay employs a two-site sandwich ELISA to quantitate MOGAT2 in samples. An antibody specific for MOGAT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MOGAT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MOGAT2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MOGAT2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: MOGAT2 contains a phosphate acyltransferase-like domain and 2 predicted N-linked glycosylation sites. Northern blot analysis revealed expression of 7.2-, 3.0-, and 1.3-kb transcripts in liver, stomach, and small intestine, with lower expression in colon and kidney. A probe specific to the truncated splice variant hybridized mainly to the 1.3-kb transcript in stomach. Immunofluorescence analysis of transiently transfected COS-7 cells showed that MGAT2 displayed a pattern consistent with localization in the endoplasmic reticulum.Substrate specificity assays of MOGAT2 demonstrated that MOGAT2 can catalyze the acylation of rac-1- and sn-2-monooleoylglycerols. MOGAT2 uses a broad range of fatty acyl-CoAs but prefers monoacylglycerols containing unsaturated fatty acids.

32,298 Da

monoacylglycerol O-acyltransferase 2

2-acylglycerol O-acyltransferase 2

2-acylglycerol O-acyltransferase 2

DC5; DGAT2L5; MGAT2; hMGAT2; hDC5  [Similar Products]

The protein encoded by this gene is an enzyme that catalyzes the synthesis of diacylglycerol from 2-monoacylglycerol and fatty acyl-CoA. The encoded protein is important in the uptake of dietary fat by the small intestine. This protein forms a complex with diacylglycerol O-acyltransferase 2 in the endoplasmic reticulum, and this complex catalyzes the synthesis of triacylglycerol. [provided by RefSeq, Dec 2015]

Catalyzes the formation of diacylglycerol from 2-monoacylglycerol and fatty acyl-CoA. Has a preference toward monoacylglycerols containing unsaturated fatty acids in an order of C18:3 > C18:2 > C18:1 > C18:0. Plays a central role in absorption of dietary fat in the small intestine by catalyzing the resynthesis of triacylglycerol in enterocytes. May play a role in diet-induced obesity.

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