Product Name
2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial (Gcat), Recombinant Protein
Full Product Name
Recombinant Mouse 2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial (Gcat)
Product Gene Name
Gcat recombinant protein
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Positions
19-416aa, Full Length of Mature Protein
Sequence
SALAQLRCIL DSELEGIRGA GTWKSERVIT SRQGPSIRVD GISGGILNFC ANNYLGLSSH PAVIQAGLQT LEEFGAGLSS TRFICGTQSI HKNLEAKIAH FHQREDAILY PSCFDANAGL FEALLTPEDA VLSDELNHAS IIDGIRLCKA HKYRYRHLDM ADLEAKLKEA QKHRLRLVAT DGAFSMDGDI APLQDICRLA AQYGALVFVD ECHATGFLGP TGRGTDELLG VMDQVTIINS TLGKALGGAS GGYTTGPEPL VSLLRQRSRP YLFSNSLPPA VVGCASKALD LLMESNAIIQ SMAAKTRRFR SKMEAAGFTV SGADHPICPV MLGDARLSSQ MADDMLKKGI FVIGFSYPVV PKGKARIRVQ ISAVHSEEDI DRCVEAFVEV GRLHGALP
3D Structure
ModBase 3D Structure for O88986
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
>85% (SDS-PAGE) (lot specific)
Form/Format
Liquid containing glycerol
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Storage Buffer
Tris-based buffer, 50% glycerol
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of Gcat recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
Gcat recombinant protein
The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. This gene encodes the second enzyme in this pathway, which then catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The encoded enzyme is considered a class II pyridoxal-phosphate-dependent aminotransferase.
NCBI/Uniprot data below describe general gene information for Gcat. It may not necessarily be applicable to this product.
NCBI Accession #
NP_038875.3
[Other Products]
NCBI GenBank Nucleotide #
NM_013847.4
[Other Products]
UniProt Primary Accession #
O88986
[Other Products]
UniProt Related Accession #
O88986[Other Products]
Molecular Weight
44,931 Da
NCBI Official Full Name
2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial isoform a
NCBI Official Synonym Full Names
glycine C-acetyltransferase (2-amino-3-ketobutyrate-coenzyme A ligase)
NCBI Official Symbol
Gcat [Similar Products]
NCBI Official Synonym Symbols
Kbl; AI526977
[Similar Products]
NCBI Protein Information
2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial
UniProt Protein Name
2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial
UniProt Synonym Protein Names
Aminoacetone synthase; Glycine acetyltransferase
UniProt Gene Name
Gcat [Similar Products]
UniProt Synonym Gene Names
Kbl; AKB ligase [Similar Products]
Research Articles on Gcat
1. Nuclear translocation of 2-amino-3-ketobutyrate coenzyme A ligase (KBL) was detected in response to cold and osmotic stresses.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.