Product Name
Connexin 43 (Cx43), Polyclonal Antibody
Full Product Name
Rabbit Anti-Human Connexin 43 (phospho Ser368) Polyclonal Antibody
Product Synonym Names
GJA1; GJAL; Gap junction alpha-1 protein; Connexin-43; Gap junction 43 kDa heart protein
Product Gene Name
anti-Cx43 antibody
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9GJX4
Specificity
Phospho-Connexin 43 (S368) Polyclonal Antibody detects endogenous levels of Connexin 43 protein only when phosphorylated at S368.
Purity/Purification
>=90% by SDS-PAGE
Affinity purified by Protein A.
Form/Format
Lyophilized or Liquid
Concentration
1 mg/mL (lot specific)
Immunogen
Synthesized peptide derived from human Connexin 43 around the phosphorylation site of S368.
Storage Buffer
0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Preparation and Storage
Upon delivery aliquot and store at -20 degree C for one year. Avoid repeated freeze/thaw cycles.
The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20 degree C.
When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-8 degree C.
Other Notes
Small volumes of anti-Cx43 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-Cx43 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-Cx43 antibody
WB: 1:500-2000
IHC: 1:100-300
ELISA: 1:20000
NCBI/Uniprot data below describe general gene information for Cx43. It may not necessarily be applicable to this product.
NCBI Accession #
CAC21170.1
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UniProt Primary Accession #
Q9GJX4
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UniProt Related Accession #
Q29101[Other Products]
NCBI Official Full Name
connexin 43, partial
NCBI Official Symbol
GJA1 [Similar Products]
NCBI Official Synonym Symbols
CX43
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NCBI Protein Information
gap junction alpha-1 protein
UniProt Protein Name
Connexin 43
UniProt Synonym Protein Names
Connexin 43
UniProt Gene Name
connexin 43 [Similar Products]
Research Articles on Cx43
1. Data show that connexin 43 (Cx43) is localized in the ooplasmic membrane through zona pellucidae and its level changes over time during culture in porcine oocytes.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.