Bifunctional heparan sulfate N deacetylase/N sulfotransferase 1 (NDST1), ELISA

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of BTA. No significant cross-reactivity or interference between BTA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BTA and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of NDST1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS756365 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Bifunctional heparan sulfate N deacetylase/N sulfotransferase 1 (NDST1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NDST1. The ELISA analytical biochemical technique of the MBS756365 kit is based on NDST1 antibody-NDST1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NDST1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NDST1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the assay: BTA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BTA antibody and an BTA-HRP conjugate. The assay sample and buffer are incubated together with BTA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BTA concentration since BTA from samples and BTA-HRP conjugate compete for the anti-BTA antibody . Since the number of sites is limited, as more sites are occupied by BTA from the sample, fewer sites are left to bind BTA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BTA concentration in each sample is interpolated from this standard curve.

Immunology

62,065 Da[Similar Products]

N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 1

bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1; NDST-1; HSNST 1; N-HSST 1; N-heparan sulfate sulfotransferase 1; glucosaminyl N-deacetylase/N-sulfotransferase 1; heparan sulfate-N-deacetylase/N-sulfotransferase; [Heparan sulfate]-glucosamine N-sulfotransferase 1

Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1

HSST; HSST1; NDST-1; N-HSST 1; HSNST 1  [Similar Products]

NDST1_HUMAN

This gene encodes a member of the heparan sulfate/heparin GlcNAc N-deacetylase/ N-sulfotransferase family. The encoded enzyme is a type II transmembrane protein that resides in the Golgi apparatus. The encoded protein catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate to nitrogen of glucosamine in heparan sulfate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

NDST1: Essential bifunctional enzyme that catalyzes both the N- deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Plays a role in determining the extent and pattern of sulfation of heparan sulfate. Compared to other NDST enzymes, its presence is absolutely required. Participates in biosynthesis of heparan sulfate that can ultimately serve as L- selectin ligands, thereby playing a role in inflammatory response. Belongs to the sulfotransferase 1 family. NDST subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; EC 2.8.2.8; Glycan Metabolism - heparan sulfate biosynthesis; Transferase; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q33.1

Cellular Component: Golgi membrane; integral to membrane

Molecular Function: deacetylase activity; [heparan sulfate]-glucosamine N-sulfotransferase activity

Biological Process: smoothened signaling pathway; fibroblast growth factor receptor signaling pathway; glycosaminoglycan metabolic process; MAPKKK cascade; pathogenesis; embryonic viscerocranium morphogenesis; respiratory gaseous exchange; glycosaminoglycan biosynthetic process; polysaccharide biosynthetic process; heparin biosynthetic process; forebrain development; midbrain development; heparan sulfate proteoglycan biosynthetic process; carbohydrate metabolic process; embryonic neurocranium morphogenesis; inflammatory response

Disease: Mental Retardation, Autosomal Recessive 46

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