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ASMT, siRNA

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产品名称: ASMT, siRNA
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简单介绍

ASMT, siRNA


ASMT, siRNA  的详细介绍
Product Name

ASMT, siRNA

Full Product Name

ASMT siRNA (Human)

Product Synonym Names
Acetylserotonin O-methyltransferase; Hydroxyindole O-methyltransferase; HIOMT
Product Gene Name

ASMT sirna

[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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OMIM
300015
3D Structure
ModBase 3D Structure for P46597
Host
Synthetic
Species Reactivity
Human
Specificity
ASMT siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ASMT gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ASMT sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for
ASMT sirna
siRNA to inhibit ASMT expression using RNA interference
Applications Tested/Suitable for ASMT sirna
RNA Interference (RNAi)
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NCBI/Uniprot data below describe general gene information for ASMT. It may not necessarily be applicable to this product.
NCBI GI #
283549144
NCBI GeneID
438
NCBI Accession #
NP_001164509.1 [Other Products]
NCBI GenBank Nucleotide #
NM_001171038.1 [Other Products]
UniProt Primary Accession #
P46597 [Other Products]
UniProt Secondary Accession #
Q16598; Q5JQ72; Q5JQ73; B2RC33[Other Products]
UniProt Related Accession #
P46597[Other Products]
Molecular Weight
41,661 Da
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NCBI Official Full Name
acetylserotonin O-methyltransferase isoform 1
NCBI Official Synonym Full Names
acetylserotonin O-methyltransferase
NCBI Official Symbol
ASMT  [Similar Products]
NCBI Official Synonym Symbols
ASMTY; HIOMT; HIOMTY
  [Similar Products]
NCBI Protein Information
acetylserotonin O-methyltransferase
UniProt Protein Name
Acetylserotonin O-methyltransferase
UniProt Synonym Protein Names
Hydroxyindole O-methyltransferase; HIOMT
Protein Family
Acetylserotonin O-methyltransferase
UniProt Gene Name
ASMT  [Similar Products]
UniProt Synonym Gene Names
HIOMT  [Similar Products]
UniProt Entry Name
ASMT_HUMAN
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NCBI Summary for ASMT
This gene belongs to the methyltransferase superfamily, and is located in the pseudoautosomal region (PAR) at the end of the short arms of the X and Y chromosomes. The encoded enzyme catalyzes the final reaction in the synthesis of melatonin, and is abundant in the pineal gland. Alternatively spliced transcript variants have been noted for this gene. [provided by RefSeq, Jan 2010]
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UniProt Comments for ASMT
ASMT: Produces melatonin (N-acetyl-5-methoxytryptamine) from N-acetylserotonin. Belongs to the methyltransferase superfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.1.1.4; Amino Acid Metabolism - tryptophan; Methyltransferase

Chromosomal Location of Human Ortholog: Xp22.3 or Yp11.3

Cellular Component: cytosol

Molecular Function: identical protein binding; protein homodimerization activity; acetylserotonin O-methyltransferase activity; O-methyltransferase activity

Biological Process: methylation; melatonin biosynthetic process; translation; indolalkylamine biosynthetic process
Research Articles on ASMT
1. results indicate that expression of sleep onset delay relates to melatonin pathway genes.
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Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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